Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605132420_5oq1 TTAGGCTGTTTGGCAGATACAACAGAGTCCTG(+CAGTGT)GATCCTCCTTGCCTCCTTCAGAAGCTCCGTA NGG 10 8 17 8 24 0 100 5 10
    240605132419_f0f6 ACGCCTACGGAGCTTCTGAAGGAGGCAAGGAG(+CAGTGT)GATCCAGGACTCTGTTGTATCTGCCAAACAG NGG 10 8 17 8 24 0 100 5 10
    240605132418_phlr ACTGTAAGAATAACAGACACCCCTTTTTGCTG(+CAGTGT)GTACCACCCTCCTCGAGTCCCCATGGTTGGC NGG 10 8 17 8 24 0 100 5 10
    240605132416_7kd6 AGGGTGCCAACCATGGGGACTCGAGGAGGGTG(+CAGTGT)GTACCAGCAAAAAGGGGTGTCTGTTATTCTT NGG 10 8 17 8 24 0 100 5 10
    240605132415_kiys TGTCTACACGTCACTACTTACAATTTTAACTG(+CAGTGT)GTACCAAGTCTGTGCCCAGAGGCAACAAGCT NGG 10 8 17 8 24 0 100 5 10
    240605132413_pr0v GGATTAGCTTGTTGCCTCTGGGCACAGACTTG(+CAGTGT)GTACCAGTTAAAATTGTAAGTAGTGACGTGT NGG 10 8 17 8 24 0 100 5 10
    240605132412_b070 CTTCATACAGCAGTCATCTGCTCGTATGTTTG(+CAGTGT)GATCCCCTTTTAAACATTCAACCCTGGAGGT NGG 10 8 17 8 24 0 100 5 10
    240605132410_czfo GATGGACCTCCAGGGTTGAATGTTTAAAAGGG(+CAGTGT)GATCCAAACATACGAGCAGATGACTGCTGTA NGG 10 8 17 8 24 0 100 5 10
    240605132409_zeph ACGATTCCCATTCTCTGTCTGTCTCGTATATG(+CAGTGT)GTACCATCTTCCAGTCACAGATCCTGACTCT NGG 10 8 17 8 24 0 100 5 10
    240605132408_mhu2 AGATGAGAGTCAGGATCTGTGACTGGAAGATG(+CAGTGT)GTACCATATACGAGACAGACAGAGAATGGGA NGG 10 8 17 8 24 0 100 5 10