Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605131215_uff8 GCTACCACAGAGCCCCCAGGAGTGTGGCCAGG(+CAGTGT)GTACCCACAGCCCTCAGACCCTCTCAATAGC NGG 10 8 17 8 24 0 100 5 10
    240605131213_i41s TTGGGAAGGAGGGCTGTGGTGTGGACCACCTG(+CAGTGT)GATCCCTATAGGAGAGACCCTGACTGTGCTC NGG 10 8 17 8 24 0 100 5 10
    240605131211_6hu3 CCACTGAGCACAGTCAGGGTCTCTCCTATAGG(+CAGTGT)GATCCAGGTGGTCCACACCACAGCCCTCCTT NGG 10 8 17 8 24 0 100 5 10
    240605131208_g7dv AGATTGGCATCAGAGGCCCCGACTCCCCCCGG(+CAGTGT)GATCCGGGAGCCTAGCCCATCCCTGGCAGCA NGG 10 8 17 8 24 0 100 5 10
    240605131205_pfnk GCGCATGCTGCCAGGGATGGGCTAGGCTCCCG(+CAGTGT)GATCCCGGGGGGAGTCGGGGCCTCTGATGCC NGG 10 8 17 8 24 0 100 5 10
    240605131204_8o2q ACATCTGCTCCTGGGGATATACGGGAGAGGAG(+CAGTGT)GTACCATAGCATCAAGTTTGGGGAGGACTGT NGG 10 8 17 8 24 0 100 5 10
    240605131203_jo8u GCACCACAGTCCTCCCCAAACTTGATGCTATG(+CAGTGT)GTACCTCCTCTCCCGTATATCCCCAGGAGCA NGG 10 8 17 8 24 0 100 5 10
    240605131202_io2y GCCTGGCAGTGCTGGGAAAGTCTCTGTAAGCG(+CAGTGT)GATCCAGAGTGTTCGAGGCACAGCCCTGGGT NGG 10 8 17 8 24 0 100 5 10
    240605131200_vddn CAGTGACCCAGGGCTGTGCCTCGAACACTCTG(+CAGTGT)GATCCGCTTACAGAGACTTTCCCAGCACTGC NGG 10 8 17 8 24 0 100 5 10
    240605131159_jlsg TGGTAGAACAAGTGTCTTGTGATCAGACACCG(+CAGTGT)GATCCCCACCTCAGGCAAAAACCACAAGGAA NGG 10 8 17 8 24 0 100 5 10