Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605131157_w718 CCGAGTTCCTTGTGGTTTTTGCCTGAGGTGGG(+CAGTGT)GATCCGGTGTCTGATCACAAGACACTTGTTC NGG 10 8 17 8 24 0 100 5 10
    240605131154_nm1e CTTATAGGTCTGAACGCAGGTCCTGCCACCCG(+CAGTGT)GTACCACCAGCCTCAGTACAGTCACTACGGC NGG 10 8 17 8 24 0 100 5 10
    240605131153_1f9x CTCAAGCCGTAGTGACTGTACTGAGGCTGGTG(+CAGTGT)GTACCGGGTGGCAGGACCTGCGTTCAGACCT NGG 10 8 17 8 24 0 100 5 10
    240605131151_dx7u CCATGCGGCTCAGCCATGCTGGTCTCTCCGAG(+CAGTGT)GTACCCAGATAGCTGCACCCCTCTGCTCCCC NGG 10 8 17 8 24 0 100 5 10
    240605131150_ugsj AGCAAGGGGAGCAGAGGGGTGCAGCTATCTGG(+CAGTGT)GTACCTCGGAGAGACCAGCATGGCTGAGCCG NGG 10 8 17 8 24 0 100 5 10
    240605131149_zdmw ACTGTCTTCAGACACAGCAGAAGAGGGCATCG(+CAGTGT)GATCCCATTACAGACTTGAGCCACCCCGTGG NGG 10 8 17 8 24 0 100 5 10
    240605131147_3ymz AGTACCCACGGGGTGGCTCAAGTCTGTAATGG(+CAGTGT)GATCCGATGCCCTCTTCTGCTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240605131146_snvn GGCACATGGCCAACCTGTTGGGTTGGTTTTTG(+CAGTGT)GTACCTAAGGGACTTTGGAAGAAGGATCAAG NGG 10 8 17 8 24 0 100 5 10
    240605131144_tpt3 GGGTTCTTGATCCTTCTTCCAAAGTCCCTTAG(+CAGTGT)GTACCAAAAACCAACCCAACAGGTTGGCCAT NGG 10 8 17 8 24 0 100 5 10
    240605131142_h8om AGAGGATACGTGGCTTGAAGCAGCTGCAGAGG(+CAGTGT)GTACCTTCAGCCTTGAGCCACACTCTTAAAT NGG 10 8 17 8 24 0 100 5 10