Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605131111_oaxb TCCTGGCTTGTGAACTGGGCCTGTTTGTAGAG(+CAGTGT)GATCCTAGAGACGTCCATGCCAGCACTCACC NGG 10 8 17 8 24 0 100 5 10
    240605131109_10i2 GTGCTGTGTTGGGAAGCTTTGGAGGTTCCTAG(+CAGTGT)GTACCTGGTATCTGGCTCCCAGCAGCAGCTC NGG 10 8 17 8 24 0 100 5 10
    240605131107_qcb9 AACAGGAGCTGCTGCTGGGAGCCAGATACCAG(+CAGTGT)GTACCTAGGAACCTCCAAAGCTTCCCAACAC NGG 10 8 17 8 24 0 100 5 10
    240605131105_3urz TGTTTAGACACATTTTACTAGGCACCTCCGTG(+CAGTGT)GATCCTTTACAGTGTTCTTGTGACAAGCTAG NGG 10 8 17 8 24 0 100 5 10
    240605131104_0nkk CCACACTAGCTTGTCACAAGAACACTGTAAAG(+CAGTGT)GATCCACGGAGGTGCCTAGTAAAATGTGTCT NGG 10 8 17 8 24 0 100 5 10
    240605131103_aop5 GCCATTTCCTTTCCCAACAGAACTCCTCATAG(+CAGTGT)GATCCCCTGTCCTTTCCCTGCCCACCTTTTG NGG 10 8 17 8 24 0 100 5 10
    240605131101_h8ph GAGTACAAAAGGTGGGCAGGGAAAGGACAGGG(+CAGTGT)GATCCTATGAGGAGTTCTGTTGGGAAAGGAA NGG 10 8 17 8 24 0 100 5 10
    240605131100_w2gi ACACAACTCCCCCACAAAAATCACTGGAGTAG(+CAGTGT)GATCCCATTAACTACACTCCTCAAATAATCA NGG 10 8 17 8 24 0 100 5 10
    240605131058_e56h CAGACTGATTATTTGAGGAGTGTAGTTAATGG(+CAGTGT)GATCCTACTCCAGTGATTTTTGTGGGGGAGT NGG 10 8 17 8 24 0 100 5 10
    240605131057_myxh AGGATATTTTCAGTTTGTAATATAAAATTTAG(+CAGTGT)GTACCCAGAACCCTCAAAAGACACAACCTCA NGG 10 8 17 8 24 0 100 5 10