Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605131009_9sl2 GGGAAGTCCTGTATGCTCTCCCTCTGGTGTTG(+CAGTGT)GTACCAGATGAATGAAAAGGAGGGCATGACC NGG 10 8 17 8 24 0 100 5 10
    240605131007_i5u0 TGTGGAAGAAGCTTGCTGCCTGAGGGCTGAGG(+CAGTGT)GATCCATAGGATCTCAATGAGGCTTGCAGTT NGG 10 8 17 8 24 0 100 5 10
    240605131005_duut CATGTAACTGCAAGCCTCATTGAGATCCTATG(+CAGTGT)GATCCCTCAGCCCTCAGGCAGCAAGCTTCTT NGG 10 8 17 8 24 0 100 5 10
    240605131004_uoep CATCAAGCAACTCTGAGTCTCTCTGGCTTCTG(+CAGTGT)GATCCTGCTACAATATATGTGGGCAGGAACA NGG 10 8 17 8 24 0 100 5 10
    240605131003_6qqn CCCTTTGTTCCTGCCCACATATATTGTAGCAG(+CAGTGT)GATCCAGAAGCCAGAGAGACTCAGAGTTGCT NGG 10 8 17 8 24 0 100 5 10
    240605131001_987e ACGGCCAAGCCATCCAAAACTAAGGGAAGTGG(+CAGTGT)GATCCCTAAGAGGTGAACCCTTAAGTGTTGT NGG 10 8 17 8 24 0 100 5 10
    240605130959_lkz6 TGGGGACAACACTTAAGGGTTCACCTCTTAGG(+CAGTGT)GATCCCACTTCCCTTAGTTTTGGATGGCTTG NGG 10 8 17 8 24 0 100 5 10
    240605130958_1hbd TTTATATTCCAATTATGCCAACAAAGGGCAAG(+CAGTGT)GTACCAGGAAAAGCATTAAACTATATTTTTT NGG 10 8 17 8 24 0 100 5 10
    240605130956_idgr GTGTTAAAAAATATAGTTTAATGCTTTTCCTG(+CAGTGT)GTACCTTGCCCTTTGTTGGCATAATTGGAAT NGG 10 8 17 8 24 0 100 5 10
    240605130955_hslh CTGTCAGTATAGTTCAAGTTTACCAGTAGAGG(+CAGTGT)GTACCTGAGAGGAACAACCACCACAGAGCTG NGG 10 8 17 8 24 0 100 5 10