Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605130953_lidk TGAGGCAGCTCTGTGGTGGTTGTTCCTCTCAG(+CAGTGT)GTACCCTCTACTGGTAAACTTGAACTATACT NGG 10 8 17 8 24 0 100 5 10
    240605130952_jexh AAAAGGCTGGAAAACAATTTTCCAAGCAAATG(+CAGTGT)GTACCAAGAAACAAGCTAGAGTAGCCATCCT NGG 10 8 17 8 24 0 100 5 10
    240605130951_h4g5 ATATTAGGATGGCTACTCTAGCTTGTTTCTTG(+CAGTGT)GTACCATTTGCTTGGAAAATTGTTTTCCAGC NGG 10 8 17 8 24 0 100 5 10
    240605130949_inql ATGAAATGAACAATTTTCTAGACAGATACCTG(+CAGTGT)GTACCAAAGTTAAATCAGGATCAGATAAAAA NGG 10 8 17 8 24 0 100 5 10
    240605130948_h0q3 TTAGATTTTTATCTGATCCTGATTTAACTTTG(+CAGTGT)GTACCAGGTATCTGTCTAGAAAATTGTTCAT NGG 10 8 17 8 24 0 100 5 10
    240605130947_gl26 ATGATCTGAAAATACTTACATATACCTGGAAG(+CAGTGT)GTACCTAAGTCATCCAACTGAATGCCAACTG NGG 10 8 17 8 24 0 100 5 10
    240605130946_vtcf ATGCCCAGTTGGCATTCAGTTGGATGACTTAG(+CAGTGT)GTACCTTCCAGGTATATGTAAGTATTTTCAG NGG 10 8 17 8 24 0 100 5 10
    240605130944_590a AAAAGGCTGGAAAACAATTTTCCAAGCAAATG(+CAGTGT)GTACCAAGAAACAAGCTAGAGTAGCCATCCT NGG 10 8 17 8 24 0 100 5 10
    240605130943_l5t7 ATATTAGGATGGCTACTCTAGCTTGTTTCTTG(+CAGTGT)GTACCATTTGCTTGGAAAATTGTTTTCCAGC NGG 10 8 17 8 24 0 100 5 10
    240605130942_w30k ATGAAGTGGACACTTTTCTAGACAGATACTAG(+CAGTGT)GTACCAAAGTTAAATCAGGATCAGATTAACG NGG 10 8 17 8 24 0 100 5 10