Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605131055_88uh ATCCATGAGGTTGTGTCTTTTGAGGGTTCTGG(+CAGTGT)GTACCTAAATTTTATATTACAAACTGAAAAT NGG 10 8 17 8 24 0 100 5 10
    240605131054_ab5y ACCACATGGTGACTCACAACCATCTGTAATGG(+CAGTGT)GATCCCATGCCCTTTTCTACTGTGTGTCTGA NGG 10 8 17 8 24 0 100 5 10
    240605131052_2lvk TGTCTTCAGACACACAGTAGAAAAGGGCATGG(+CAGTGT)GATCCCATTACAGATGGTTGTGAGTCACCAT NGG 10 8 17 8 24 0 100 5 10
    240605131051_vwyx TTACAGAGACCAGGCAGCCATGGACAGTGAGG(+CAGTGT)GTACCCAATGTAACCACAAGGCCAGGCAGGT NGG 10 8 17 8 24 0 100 5 10
    240605131049_yvy5 CTGGGACCTGCCTGGCCTTGTGGTTACATTGG(+CAGTGT)GTACCCTCACTGTCCATGGCTGCCTGGTCTC NGG 10 8 17 8 24 0 100 5 10
    240605131048_ppfg AAAGGACACTTTCCCATTCCCTGTACCACCAG(+CAGTGT)GTACCAGATATGAGCTGGATATATGGCCAAG NGG 10 8 17 8 24 0 100 5 10
    240605131046_bqbb TGAGGCTTGGCCATATATCCAGCTCATATCTG(+CAGTGT)GTACCTGGTGGTACAGGGAATGGGAAAGTGT NGG 10 8 17 8 24 0 100 5 10
    240605131044_u9ub TGTAAAACAAGCTCTGACCAGCAACTCCCAGG(+CAGTGT)GTACCCACTTCCGCATCACCCACCTGGCTGT NGG 10 8 17 8 24 0 100 5 10
    240605131042_uv80 TCATCACAGCCAGGTGGGTGATGCGGAAGTGG(+CAGTGT)GTACCCTGGGAGTTGCTGGTCAGAGCTTGTT NGG 10 8 17 8 24 0 100 5 10
    240605131041_eyim CACCAAGAGCAGAGGTACTGCTGGGATTATAG(+CAGTGT)GATCCTACAATTCTCACTGGACCTGGAAGGG NGG 10 8 17 8 24 0 100 5 10