Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605131024_o9jr GGCCAAACAGGGCAGCTCAATTAGGGGAAGAG(+CAGTGT)GATCCACAGGCAGGCAACTGATTTAGGGACA NGG 10 8 17 8 24 0 100 5 10
    240605131023_fy6z ACCTGGTTGTTATTTTCCCATCACCTTAAAGG(+CAGTGT)GATCCTCATTAGCATCTAAGATCCTACTGCT NGG 10 8 17 8 24 0 100 5 10
    240605131021_84zg AGAGGAGCAGTAGGATCTTAGATGCTAATGAG(+CAGTGT)GATCCCTTTAAGGTGATGGGAAAATAACAAC NGG 10 8 17 8 24 0 100 5 10
    240605131020_znk3 GAAGCCTGAAGTTCACCCCCAGGCAGAAGGTG(+CAGTGT)GTACCCTGGACATCCAGTCTGCCTCAGAGCA NGG 10 8 17 8 24 0 100 5 10
    240605131018_3ya9 TCCTCTGCTCTGAGGCAGACTGGATGTCCAGG(+CAGTGT)GTACCACCTTCTGCCTGGGGGTGAACTTCAG NGG 10 8 17 8 24 0 100 5 10
    240605131017_trgh GACGGGAGTTGGTTCTCTCCTTCCACTGTGTG(+CAGTGT)GATCCTGAAGAGCAAGCTCAGGACCTCAGGT NGG 10 8 17 8 24 0 100 5 10
    240605131015_5s09 CATCCACCTGAGGTCCTGAGCTTGCTCTTCAG(+CAGTGT)GATCCACACAGTGGAAGGAGAGAACCAACTC NGG 10 8 17 8 24 0 100 5 10
    240605131014_jllt AGAGGCATAGCATAATTGGGTGTTTACTAAGG(+CAGTGT)GATCCCTGGTGGTGGGTTACACAATAGACTG NGG 10 8 17 8 24 0 100 5 10
    240605131011_k8l0 AATTCCAGTCTATTGTGTAACCCACCACCAGG(+CAGTGT)GATCCCTTAGTAAACACCCAATTATGCTATG NGG 10 8 17 8 24 0 100 5 10
    240605131010_qwiq CGAGTGGTCATGCCCTCCTTTTCATTCATCTG(+CAGTGT)GTACCAACACCAGAGGGAGAGCATACAGGAC NGG 10 8 17 8 24 0 100 5 10