Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605130856_2b2s ACGGAAGGAGAGATTTCAGCGCTGACCACTTG(+CAGTGT)GATCCAATGATAGCTCAGTTGGTGCATGCAA NGG 10 8 17 8 24 0 100 5 10
    240605130854_ptcj CCCTTCCTGAGGACTGTAACTCCAGCTCTGGG(+CAGTGT)GATCCAGTGTCCTCTCGTGCCCTCCAGAGCA NGG 10 8 17 8 24 0 100 5 10
    240605130852_x35u GTGCATGCTCTGGAGGGCACGAGAGGACACTG(+CAGTGT)GATCCCCAGAGCTGGAGTTACAGTCCTCAGG NGG 10 8 17 8 24 0 100 5 10
    240605130851_d4cw CGCTGCTTGTATATGAAAATAAAAGGCATACG(+CAGTGT)GTACCCCTGAGACAAAAGCCGAGGGGGGTGT NGG 10 8 17 8 24 0 100 5 10
    240605130849_ttxh GAATAACACCCCCCTCGGCTTTTGTCTCAGGG(+CAGTGT)GTACCGTATGCCTTTTATTTTCATATACAAG NGG 10 8 17 8 24 0 100 5 10
    240605130848_8okv CTGTGAGGTCAAGGCATCGAGCGGCATAGTAG(+CAGTGT)GATCCTGTCTCAAGCACACCGGCTAGAGTGG NGG 10 8 17 8 24 0 100 5 10
    240605130847_2tpp AGCTACCACTCTAGCCGGTGTGCTTGAGACAG(+CAGTGT)GATCCTACTATGCCGCTCGATGCCTTGACCT NGG 10 8 17 8 24 0 100 5 10
    240605130845_vpii CAGGAGACCAATCCTGCCAGGTGGGCAAGCTG(+CAGTGT)GATCCGGGTAACAGCTATTTGCAAAGAACAA NGG 10 8 17 8 24 0 100 5 10
    240605130843_16gx GTTGGTTGTTCTTTGCAAATAGCTGTTACCCG(+CAGTGT)GATCCAGCTTGCCCACCTGGCAGGATTGGTC NGG 10 8 17 8 24 0 100 5 10
    240605130842_u5pv GCACTTCTTGCTCTAGCATCTTCTTCAAGTGG(+CAGTGT)GATCCAGCAAAGGAGGCTGAGATGTCTAAGG NGG 10 8 17 8 24 0 100 5 10