Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605130813_oser AGGAAGAGTTCATGTAGTGTCAGGCACTGTTG(+CAGTGT)GTACCCTCCCCCTTTAAGATTTATTTTTATT NGG 10 8 17 8 24 0 100 5 10
    240605130810_al5i TTTAGGACTGACACCCTGAGTTTAGTCCCTGG(+CAGTGT)GATCCTCGTGGTGGGAGAGAGAACTGACTTC NGG 10 8 17 8 24 0 100 5 10
    240605130808_ykar GTGTGGAAGTCAGTTCTCTCTCCCACCACGAG(+CAGTGT)GATCCCAGGGACTAAACTCAGGGTGTCAGTC NGG 10 8 17 8 24 0 100 5 10
    240605130807_g3ot CCTGCTTCGACTTGACTTGCCAGGGCGGGTTG(+CAGTGT)GTACCCATGGGGAGGCCTCCCCTTCTCAGAA NGG 10 8 17 8 24 0 100 5 10
    240605130805_eb48 TTTCCTTCTGAGAAGGGGAGGCCTCCCCATGG(+CAGTGT)GTACCAACCCGCCCTGGCAAGTCAAGTCGAA NGG 10 8 17 8 24 0 100 5 10
    240605130804_bc6k CTTTTTGAAAGTAAATACTTTATGTAGCAAAG(+CAGTGT)GTACCTGCATAATTGTTGGCACCTTTTTATA NGG 10 8 17 8 24 0 100 5 10
    240605130802_et9d TAGTGTATAAAAAGGTGCCAACAATTATGCAG(+CAGTGT)GTACCTTTGCTACATAAAGTATTTACTTTCA NGG 10 8 17 8 24 0 100 5 10
    240605130801_oy2a CTTTTTGAAAGTAAATACTTTATGTAGCAAAG(+CAGTGT)GTACCTGCATAATAGTGGGCACCTTTTTATA NGG 10 8 17 8 24 0 100 5 10
    240605130800_05rb TAGTGTATAAAAAGGTGCCCACTATTATGCAG(+CAGTGT)GTACCTTTGCTACATAAAGTATTTACTTTCA NGG 10 8 17 8 24 0 100 5 10
    240605130759_n6tk ACAAAAAACCAACTAAGAAAGTTAATGATAAG(+CAGTGT)GTACCATTGATGGTGAAATGTTTGGTCAAAC NGG 10 8 17 8 24 0 100 5 10