Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605130741_gopm TCTTAGCTACAGGGTGGATCTGGCTGTGCCTG(+CAGTGT)GATCCGCCTGGGTCTATTCTTCAGCCCTGAA NGG 10 8 17 8 24 0 100 5 10
    240605130740_79yv TTGAAGTCCCACTGTTGGCAGTTTTGGCAGTG(+CAGTGT)GTACCTAACTCATCGTGTTTGCATTCTTGTT NGG 10 8 17 8 24 0 100 5 10
    240605130739_nilp AACTTAACAAGAATGCAAACACGATGAGTTAG(+CAGTGT)GTACCACTGCCAAAACTGCCAACAGTGGGAC NGG 10 8 17 8 24 0 100 5 10
    240605130738_hhic AGAAGTCAATGCTGTTCAGATGAAGTCTCTAG(+CAGTGT)GTACCAAACCAACATGTTCTCTTTTATCAAT NGG 10 8 17 8 24 0 100 5 10
    240605130737_1qbp CGAGCATTGATAAAAGAGAACATGTTGGTTTG(+CAGTGT)GTACCTAGAGACTTCATCTGAACAGCATTGA NGG 10 8 17 8 24 0 100 5 10
    240605130735_8rgv GCAACTGGAGGGGCCGTGAGGAGGAGGAGCTG(+CAGTGT)GTACCTTGCTAGGCGGAAGCAGGCACTGTGA NGG 10 8 17 8 24 0 100 5 10
    240605130734_g5wo CTCTGTCACAGTGCCTGCTTCCGCCTAGCAAG(+CAGTGT)GTACCAGCTCCTCCTCCTCACGGCCCCTCCA NGG 10 8 17 8 24 0 100 5 10
    240605130733_d12z TCTTCAGACAATGTTGCTTTGCTGCCTGTTTG(+CAGTGT)GATCCTTGTACATTTTACTTTACAAGAGAGA NGG 10 8 17 8 24 0 100 5 10
    240605130732_qdiv TGGAATCTCTCTTGTAAAGTAAAATGTACAAG(+CAGTGT)GATCCAAACAGGCAGCAAAGCAACATTGTCT NGG 10 8 17 8 24 0 100 5 10
    240605130731_x5qk AATTATCTGCAACCCCCGTCCTAGAGGATCTG(+CAGTGT)GTACCCACCTATGTGGGCATTGCAAGCACGG NGG 10 8 17 8 24 0 100 5 10