Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605131256_0np1 GCAAGTCAGGGTTTTGAAAACAGTAACAAAAG(+CAGTGT)GTACCCAAAACTCAGGCTTAACCAAATACTG NGG 10 8 17 8 24 0 100 5 10
    240605131254_sm5z CCCAAGCCAAGCCTCAGCCTGGGCTTTCCCAG(+CAGTGT)GATCCTGTTAGTGAGCAGGGCTCAGCCACTC NGG 10 8 17 8 24 0 100 5 10
    240605131253_v7xc GGTCTGAGTGGCTGAGCCCTGCTCACTAACAG(+CAGTGT)GATCCTGGGAAAGCCCAGGCTGAGGCTTGGC NGG 10 8 17 8 24 0 100 5 10
    240605131251_ax3g TGCAGCCACTGCCAGAGCTGACCTTGACTGGG(+CAGTGT)GATCCTTGTCTGGGACCCCATCACGCTGGTC NGG 10 8 17 8 24 0 100 5 10
    240605131250_0ntw TAGAGGACCAGCGTGATGGGGTCCCAGACAAG(+CAGTGT)GATCCCCAGTCAAGGTCAGCTCTGGCAGTGG NGG 10 8 17 8 24 0 100 5 10
    240605131248_aopz TAACTTGTTTCATTTCTTTTCAGATCCCTAAG(+CAGTGT)GATCCCCCCACCCCCCAATGCCGCTCCAACT NGG 10 8 17 8 24 0 100 5 10
    240605131245_nmhr TTCAGAGTTGGAGCGGCATTGGGGGGTGGGGG(+CAGTGT)GATCCTTAGGGATCTGAAAAGAAATGAAACA NGG 10 8 17 8 24 0 100 5 10
    240605131244_utex TTCAGAGCCAGCACATCACCATCTGTTTCTGG(+CAGTGT)GTACCTACCTTATGCCTACCAGAGGGCTCGT NGG 10 8 17 8 24 0 100 5 10
    240605131242_e7ev ATAAAACGAGCCCTCTGGTAGGCATAAGGTAG(+CAGTGT)GTACCCAGAAACAGATGGTGATGTGCTGGCT NGG 10 8 17 8 24 0 100 5 10
    240605131241_3931 GGTGTCTGACCCTCCACCACCTCACCTTGTTG(+CAGTGT)GTACCTCCCTCCCTGAATTCCTGAGCCAGCA NGG 10 8 17 8 24 0 100 5 10