Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605130827_oelt CTCACTGGAATACTGTTCAAGCTGAGGTCCAG(+CAGTGT)GATCCAAACTTGGTGGGTGGGAGGGGTTTCC NGG 10 8 17 8 24 0 100 5 10
    240605130825_1kny AAATGCTATGACTTTAAGGGTCTTGGGGGGAG(+CAGTGT)GATCCATGACCTAAACACAGTCGTTTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240605130824_cacd AAAAAAAAAAAAACGACTGTGTTTAGGTCATG(+CAGTGT)GATCCTCCCCCCAAGACCCTTAAAGTCATAG NGG 10 8 17 8 24 0 100 5 10
    240605130823_1k0t AATAAAAGTAAGAAAATATGCTCAATGCTGAG(+CAGTGT)GTACCTTAAAAAAACAAAAGTTTATAAAATA NGG 10 8 17 8 24 0 100 5 10
    240605130821_0xmc TAAAATATTTTATAAACTTTTGTTTTTTTAAG(+CAGTGT)GTACCTCAGCATTGAGCATATTTTCTTACTT NGG 10 8 17 8 24 0 100 5 10
    240605130820_1ogh TGCTCAACCTTATTTATATCACTGACTGGCTG(+CAGTGT)GATCCAAAGTCACACCTCCACATATTAGTGA NGG 10 8 17 8 24 0 100 5 10
    240605130819_ykud TTTATTCACTAATATGTGGAGGTGTGACTTTG(+CAGTGT)GATCCAGCCAGTCAGTGATATAAATAAGGTT NGG 10 8 17 8 24 0 100 5 10
    240605130818_c1do ACTGTGGGTGCTTGAGGAGGCCAGAGGCATAG(+CAGTGT)GATCCCTCTGGAGCATGAATTACAAGTGGCT NGG 10 8 17 8 24 0 100 5 10
    240605130816_d09l CTGACAGCCACTTGTAATTCATGCTCCAGAGG(+CAGTGT)GATCCTATGCCTCTGGCCTCCTCAAGCACCC NGG 10 8 17 8 24 0 100 5 10
    240605130814_p5ne CCAACAATAAAAATAAATCTTAAAGGGGGAGG(+CAGTGT)GTACCAACAGTGCCTGACACTACATGAACTC NGG 10 8 17 8 24 0 100 5 10