Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605130644_b4ds AAATGCATAGTTACCAGGAAGGTATGTACCGG(+CAGTGT)GTACCAAGGACAGGTAAAGATAACCTGTAGT NGG 10 8 17 8 24 0 100 5 10
    240605130643_1jyl AATCAAACCTGGGGAGAGATCCATGCGGCACG(+CAGTGT)GATCCTGGGTTTAAGAGCAGGAAGGTCAGAA NGG 10 8 17 8 24 0 100 5 10
    240605130641_nbfw ACTGCTTCTGACCTTCCTGCTCTTAAACCCAG(+CAGTGT)GATCCGTGCCGCATGGATCTCTCCCCAGGTT NGG 10 8 17 8 24 0 100 5 10
    240605130640_d1dm AGTGATAAAAAGAAAACAACCAAAGAAAATTG(+CAGTGT)GTACCTACTCTTCTGCATAAGAAGTGTGTCT NGG 10 8 17 8 24 0 100 5 10
    240605130638_hxka TATCTAGACACACTTCTTATGCAGAAGAGTAG(+CAGTGT)GTACCAATTTTCTTTGGTTGTTTTCTTTTTA NGG 10 8 17 8 24 0 100 5 10
    240605130637_4pcj TGAAGGTCAAACAGGTTCTCATTGCCCTGCAG(+CAGTGT)GTACCTTGCTCTGGACTGCTTCTGTATGGGG NGG 10 8 17 8 24 0 100 5 10
    240605130636_zdu2 AGTCACCCCATACAGAAGCAGTCCAGAGCAAG(+CAGTGT)GTACCTGCAGGGCAATGAGAACCTGTTTGAC NGG 10 8 17 8 24 0 100 5 10
    240605130634_r8wb GGGCTGCCTGACAGCGTCACAGCTGGGAATGG(+CAGTGT)GATCCCAAAATCGTTTCCTGTTTGCATCTTC NGG 10 8 17 8 24 0 100 5 10
    240605130633_1rf5 TAGAGGAAGATGCAAACAGGAAACGATTTTGG(+CAGTGT)GATCCCATTCCCAGCTGTGACGCTGTCAGGC NGG 10 8 17 8 24 0 100 5 10
    240605130631_2jz8 CCTCTAGTGACTCCCCACTGACAGATCATTGG(+CAGTGT)GATCCATTGTGCATCACGATGGTGATGGAGG NGG 10 8 17 8 24 0 100 5 10