Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605131104_0nkk CCACACTAGCTTGTCACAAGAACACTGTAAAG(+CAGTGT)GATCCACGGAGGTGCCTAGTAAAATGTGTCT NGG 10 8 17 8 24 0 100 5 10
    240605131103_aop5 GCCATTTCCTTTCCCAACAGAACTCCTCATAG(+CAGTGT)GATCCCCTGTCCTTTCCCTGCCCACCTTTTG NGG 10 8 17 8 24 0 100 5 10
    240605131101_h8ph GAGTACAAAAGGTGGGCAGGGAAAGGACAGGG(+CAGTGT)GATCCTATGAGGAGTTCTGTTGGGAAAGGAA NGG 10 8 17 8 24 0 100 5 10
    240605131100_w2gi ACACAACTCCCCCACAAAAATCACTGGAGTAG(+CAGTGT)GATCCCATTAACTACACTCCTCAAATAATCA NGG 10 8 17 8 24 0 100 5 10
    240605131058_e56h CAGACTGATTATTTGAGGAGTGTAGTTAATGG(+CAGTGT)GATCCTACTCCAGTGATTTTTGTGGGGGAGT NGG 10 8 17 8 24 0 100 5 10
    240605131057_myxh AGGATATTTTCAGTTTGTAATATAAAATTTAG(+CAGTGT)GTACCCAGAACCCTCAAAAGACACAACCTCA NGG 10 8 17 8 24 0 100 5 10
    240605131055_88uh ATCCATGAGGTTGTGTCTTTTGAGGGTTCTGG(+CAGTGT)GTACCTAAATTTTATATTACAAACTGAAAAT NGG 10 8 17 8 24 0 100 5 10
    240605131054_ab5y ACCACATGGTGACTCACAACCATCTGTAATGG(+CAGTGT)GATCCCATGCCCTTTTCTACTGTGTGTCTGA NGG 10 8 17 8 24 0 100 5 10
    240605131052_2lvk TGTCTTCAGACACACAGTAGAAAAGGGCATGG(+CAGTGT)GATCCCATTACAGATGGTTGTGAGTCACCAT NGG 10 8 17 8 24 0 100 5 10
    240605131051_vwyx TTACAGAGACCAGGCAGCCATGGACAGTGAGG(+CAGTGT)GTACCCAATGTAACCACAAGGCCAGGCAGGT NGG 10 8 17 8 24 0 100 5 10