Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605130543_znae TTACAAAATTCTATTAGAAAAGAAGAAGACAG(+CAGTGT)GATCCCGTTTGTTATGTTACAGACTTAGACA NGG 10 8 17 8 24 0 100 5 10
    240605130540_6nak ACGGGCTAATCTGCTTGTCATGAAGCCCCAAG(+CAGTGT)GATCCCTCTCTGCTTCTCCACTGCTGACATT NGG 10 8 17 8 24 0 100 5 10
    240605130539_0ydf GTTGCAATGTCAGCAGTGGAGAAGCAGAGAGG(+CAGTGT)GATCCTTGGGGCTTCATGACAAGCAGATTAG NGG 10 8 17 8 24 0 100 5 10
    240605130538_flug TAAAAAGTCTTGCCTCTGAAGACCTTTTAAAG(+CAGTGT)GTACCATGAGTAGGAATTGGGAAGAATTGTT NGG 10 8 17 8 24 0 100 5 10
    240605130536_656b ATTGTAACAATTCTTCCCAATTCCTACTCATG(+CAGTGT)GTACCTTTAAAAGGTCTTCAGAGGCAAGACT NGG 10 8 17 8 24 0 100 5 10
    240605130535_oqw0 TTTCTTAATGTTCTAATTATAATGAAAGTCGG(+CAGTGT)GATCCCATGTCACACTTTGGGAAACCTTTGA NGG 10 8 17 8 24 0 100 5 10
    240605130533_6q06 CTAAATCAAAGGTTTCCCAAAGTGTGACATGG(+CAGTGT)GATCCCGACTTTCATTATAATTAGAACATTA NGG 10 8 17 8 24 0 100 5 10
    240605130532_4c9t GTCTGTTTTACACTAGGTTCTAGAAGTAATTG(+CAGTGT)GATCCCAAAGTTCTTTGGCTATTATAAAGTA NGG 10 8 17 8 24 0 100 5 10
    240605130530_0plz GTCTATACTTTATAATAGCCAAAGAACTTTGG(+CAGTGT)GATCCAATTACTTCTAGAACCTAGTGTAAAA NGG 10 8 17 8 24 0 100 5 10
    240605130528_zi67 GAAAACGAGACTCTATATGCCTTTTGACCATG(+CAGTGT)GATCCTGTGTTTGAATGACAGGCATTAATTT NGG 10 8 17 8 24 0 100 5 10