Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605130630_zeq2 TTCACCCTCCATCACCATCGTGATGCACAATG(+CAGTGT)GATCCCAATGATCTGTCAGTGGGGAGTCACT NGG 10 8 17 8 24 0 100 5 10
    240605130628_m18x AAAAGAAAGAAAGAAACTGTTCTTTTCATTTG(+CAGTGT)GTACCATAGTGTGAAGAGCTGGGAGCAATGA NGG 10 8 17 8 24 0 100 5 10
    240605130627_r5af AACAGTCATTGCTCCCAGCTCTTCACACTATG(+CAGTGT)GTACCAAATGAAAAGAACAGTTTCTTTCTTT NGG 10 8 17 8 24 0 100 5 10
    240605130626_lpq7 CAAGCTCAGGTTCTCCCATCCAGGGGGAAGTG(+CAGTGT)GTACCCACGTTGACTAGAATGGGAAGATGTT NGG 10 8 17 8 24 0 100 5 10
    240605130624_0qg9 TTGGGAACATCTTCCCATTCTAGTCAACGTGG(+CAGTGT)GTACCACTTCCCCCTGGATGGGAGAACCTGA NGG 10 8 17 8 24 0 100 5 10
    240605130623_xe5i ATGTACTTCATGAGAAAATTCAACAGCAGCTG(+CAGTGT)GTACCGGATTCCAAGAAGGAAGGAAGCACAC NGG 10 8 17 8 24 0 100 5 10
    240605130621_a2he CACCCGTGTGCTTCCTTCCTTCTTGGAATCCG(+CAGTGT)GTACCAGCTGCTGTTGAATTTTCTCATGAAG NGG 10 8 17 8 24 0 100 5 10
    240605130619_md8n AAGTCACAGACTTTAAAAGTAGGTATGGGCGG(+CAGTGT)GATCCATCCAAGCCATCACTGGTCAGGAGAG NGG 10 8 17 8 24 0 100 5 10
    240605130618_eg8m GATGTCTCTCCTGACCAGTGATGGCTTGGATG(+CAGTGT)GATCCCGCCCATACCTACTTTTAAAGTCTGT NGG 10 8 17 8 24 0 100 5 10
    240605130616_54t0 GTCCCTGTGCTGATGGGGAGGAGGAGGGCAGG(+CAGTGT)GTACCGGGAGCAGGTAGCAGAAGGAGCCTCT NGG 10 8 17 8 24 0 100 5 10