Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605130454_zp2s CTGCCACTTGTAAAGGCGGAGCCATGTTAATG(+CAGTGT)GATCCTGCCCTGCGTTAATGTATGATACATT NGG 10 8 17 8 24 0 100 5 10
    240605130452_tgwv ACGAGTCATTGCTTCTGGTTTGTCCTAACAGG(+CAGTGT)GTACCTGACAGGAAATTGCTAGCTCTGGCAG NGG 10 8 17 8 24 0 100 5 10
    240605130451_v77s AGTTTCTGCCAGAGCTAGCAATTTCCTGTCAG(+CAGTGT)GTACCCTGTTAGGACAAACCAGAAGCAATGA NGG 10 8 17 8 24 0 100 5 10
    240605130450_dfdv GTAGGGTGTTCCTCCCAGTTCAGGTAGTGTTG(+CAGTGT)GATCCTGAGTTGTGGCTGGGATAACTCTTCA NGG 10 8 17 8 24 0 100 5 10
    240605130449_zfkw AATCCTGAAGAGTTATCCCAGCCACAACTCAG(+CAGTGT)GATCCAACACTACCTGAACTGGGAGGAACAC NGG 10 8 17 8 24 0 100 5 10
    240605130448_yz28 TTACTCTCAAGGCTTCATGATCGACAATGAAG(+CAGTGT)GATCCTGCAGGAACGGAGGTGGTGGTGGCAC NGG 10 8 17 8 24 0 100 5 10
    240605130447_nwd8 CATCTGTGCCACCACCACCTCCGTTCCTGCAG(+CAGTGT)GATCCTTCATTGTCGATCATGAAGCCTTGAG NGG 10 8 17 8 24 0 100 5 10
    240605130444_gsqo AGACTGCAGCATTGGAGGCTCAGGGACCTTGG(+CAGTGT)GTACCTAAAGACAGACCTTGGGAAGCCCAAG NGG 10 8 17 8 24 0 100 5 10
    240605130443_ef79 CTTCCCTTGGGCTTCCCAAGGTCTGTCTTTAG(+CAGTGT)GTACCCAAGGTCCCTGAGCCTCCAATGCTGC NGG 10 8 17 8 24 0 100 5 10
    240605130442_u5i0 AAATCATACCAAATGCACTCCACACTTTTTAG(+CAGTGT)GTACCTCCTTCCATCCACAGAAAGTAGGCCA NGG 10 8 17 8 24 0 100 5 10