Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605130920_zsxa CCAGTTCAGAGGAGATTGTCTGGCTGCCTTTG(+CAGTGT)GATCCTCCAGCACCAAATCTGCCTGCACAAT NGG 10 8 17 8 24 0 100 5 10
    240605130919_zoa9 TTCTTTGGGTTTGTCGTTGGATTTGGAGCGAG(+CAGTGT)GATCCATTTTCTCTTTACTCTGTCATTTGTC NGG 10 8 17 8 24 0 100 5 10
    240605130918_0p4p ACAGTGACAAATGACAGAGTAAAGAGAAAATG(+CAGTGT)GATCCTCGCTCCAAATCCAACGACAAACCCA NGG 10 8 17 8 24 0 100 5 10
    240605130916_2ou2 TCACCTAGAGACCCTGTGTAGATTTAACCAAG(+CAGTGT)GTACCAAGAAAGTCCTGATGTTACCACAAGG NGG 10 8 17 8 24 0 100 5 10
    240605130915_l9mm GTGGTCCTTGTGGTAACATCAGGACTTTCTTG(+CAGTGT)GTACCTTGGTTAAATCTACACAGGGTCTCTA NGG 10 8 17 8 24 0 100 5 10
    240605130914_jk72 TGCAATAGCTACAGACCAAACTGGCACAAACG(+CAGTGT)GATCCTGTCCAACTGCATTGGTTTTCAAGGT NGG 10 8 17 8 24 0 100 5 10
    240605130912_k1f1 TTTGTACCTTGAAAACCAATGCAGTTGGACAG(+CAGTGT)GATCCGTTTGTGCCAGTTTGGTCTGTAGCTA NGG 10 8 17 8 24 0 100 5 10
    240605130910_vzn7 GTCCTCCAGGCTCCACCAAGACACACACCGGG(+CAGTGT)GTACCACATGGTCCATTTCATGTGATCTATT NGG 10 8 17 8 24 0 100 5 10
    240605130908_njq6 AGAGTAATAGATCACATGAAATGGACCATGTG(+CAGTGT)GTACCCCGGTGTGTGTCTTGGTGGAGCCTGG NGG 10 8 17 8 24 0 100 5 10
    240605130906_fo6z ACCTCGTCTTCCCCTCCTTCAGGTTAGCCTGG(+CAGTGT)GTACCAGTAGCACGTCTCCTAGTGGCCGGCA NGG 10 8 17 8 24 0 100 5 10