Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605130322_o2vr TTTATTTTTAGTCTTTATAGAAATCTACTGCG(+CAGTGT)GATCCTACAGAGTCTGAGGACTAACCCTTGC NGG 10 8 17 8 24 0 100 5 10
    240605130321_69cg TGGGTGCACTTTGTCATGTAGTGGTCTCGGAG(+CAGTGT)GATCCGCCAGCAATGGAAAGGCTTGGCACTC NGG 10 8 17 8 24 0 100 5 10
    240605130319_v1rx ACACTGAGTGCCAAGCCTTTCCATTGCTGGCG(+CAGTGT)GATCCTCCGAGACCACTACATGACAAAGTGC NGG 10 8 17 8 24 0 100 5 10
    240605130318_wedg ATGCTGTGGTAAGTACTACATCTACAATTTTG(+CAGTGT)GTACCCGAACTGGTGCTTTAGAAATGCGGGG NGG 10 8 17 8 24 0 100 5 10
    240605130316_8x3l AAAAACCCCGCATTTCTAAAGCACCAGTTCGG(+CAGTGT)GTACCAAAATTGTAGATGTAGTACTTACCAC NGG 10 8 17 8 24 0 100 5 10
    240605130315_j5ec GCGTGTTCCAAAGATGGCTCCGTGTTGCAATG(+CAGTGT)GTACCCACCGCTTCACTAATGCCTCCAAGTG NGG 10 8 17 8 24 0 100 5 10
    240605130313_yrbg AGCCACACTTGGAGGCATTAGTGAAGCGGTGG(+CAGTGT)GTACCATTGCAACACGGAGCCATCTTTGGAA NGG 10 8 17 8 24 0 100 5 10
    240605130312_g2hp GTGCTTCATCCTAGAGAAGGCGACCGGTCTGG(+CAGTGT)GATCCATGCATATGGAAAGGCTGGTCTTACT NGG 10 8 17 8 24 0 100 5 10
    240605130310_pcls ACCTGAGTAAGACCAGCCTTTCCATATGCATG(+CAGTGT)GATCCCAGACCGGTCGCCTTCTCTAGGATGA NGG 10 8 17 8 24 0 100 5 10
    240605130309_wx59 GCAAACAGCAAGAGGCTTTATTGGGAGCACGG(+CAGTGT)GTACCCGGGCGACGCAGTCTATCGGAGGACT NGG 10 8 17 8 24 0 100 5 10