Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605130252_7mp7 GGGGAGCTGTGCGGCAGTGAGGAAGACGAGCG(+CAGTGT)GATCCCATAGCCCCTCCTGCCACGTGAGATT NGG 10 8 17 8 24 0 100 5 10
    240605130251_riq3 AATCAACCAATCATATGAAAAAGAACTTTGTG(+CAGTGT)GTACCTAGAGGGGCAGAGCCTGCAAGGTGGG NGG 10 8 17 8 24 0 100 5 10
    240605130249_7p8g CAGGGCCCACCTTGCAGGCTCTGCCCCTCTAG(+CAGTGT)GTACCACAAAGTTCTTTTTCATATGATTGGT NGG 10 8 17 8 24 0 100 5 10
    240605130248_shdd CCCCTGGGAAAGAATTTTTTAAAAGGGCTAGG(+CAGTGT)GTACCATTCATCCAATATTTATAGAAAATGG NGG 10 8 17 8 24 0 100 5 10
    240605130247_faj8 TTCTGCCATTTTCTATAAATATTGGATGAATG(+CAGTGT)GTACCCTAGCCCTTTTAAAAAATTCTTTCCC NGG 10 8 17 8 24 0 100 5 10
    240605130245_0c92 AATACACATGTGCCAAAACTTGTCTGTAGAGG(+CAGTGT)GATCCGACTCATGGGCCGTGTGCGTGCAGTA NGG 10 8 17 8 24 0 100 5 10
    240605130244_4e82 CTGCCTACTGCACGCACACGGCCCATGAGTCG(+CAGTGT)GATCCCTCTACAGACAAGTTTTGGCACATGT NGG 10 8 17 8 24 0 100 5 10
    240605130242_xpdz AATACACATGTGCCAAAACTTGTCTGTAGAGG(+CAGTGT)GATCCGACTCATGGGCCGTGTGCGTGCAGTA NGG 10 8 17 8 24 0 100 5 10
    240605130241_nehu CTGCCTACTGCACGCACACGGCCCATGAGTCG(+CAGTGT)GATCCCTCTACAGACAAGTTTTGGCACATGT NGG 10 8 17 8 24 0 100 5 10
    240605130239_xf7y TGGCCACTCCTCCCCACCACAGAGTGTCCTGG(+CAGTGT)GATCCCAGAGGAGACTCATCCTGCAGCGTGA NGG 10 8 17 8 24 0 100 5 10