Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605130221_lctw AGACAAATGCCTTCCTCAAGTTTAGAAGGAAG(+CAGTGT)GTACCATATGAATCCGTGAAGGAGGCCCCAG NGG 10 8 17 8 24 0 100 5 10
    240605130219_y5gg ACTTCTCCCTGTCAGCCCCAGCTGGCCCATGG(+CAGTGT)GATCCTGGGACAGCCGACTCATGACCCACCT NGG 10 8 17 8 24 0 100 5 10
    240605130216_h6ck TGTCCAGGTGGGTCATGAGTCGGCTGTCCCAG(+CAGTGT)GATCCCATGGGCCAGCTGGGGCTGACAGGGA NGG 10 8 17 8 24 0 100 5 10
    240605130215_ottl AGGGCAGCATCTCCTGGGAAAACGAACTAGAG(+CAGTGT)GATCCAGAGCCTTGTGGCCCTTTCACGGTCA NGG 10 8 17 8 24 0 100 5 10
    240605130214_ljr6 TTCTCTGACCGTGAAAGGGCCACAAGGCTCTG(+CAGTGT)GATCCTCTAGTTCGTTTTCCCAGGAGATGCT NGG 10 8 17 8 24 0 100 5 10
    240605130212_nt0g GGCTCCCCTGCCTCAGTCTGCAAGGCTACTTG(+CAGTGT)GTACCTCCTCACGCTCCGAGAAGTCTCACAC NGG 10 8 17 8 24 0 100 5 10
    240605130211_7hzv GGTAAGTGTGAGACTTCTCGGAGCGTGAGGAG(+CAGTGT)GTACCAAGTAGCCTTGCAGACTGAGGCAGGG NGG 10 8 17 8 24 0 100 5 10
    240605130210_ldhu GGAGGGGCACCCCACTCCTCTATGGCAACTGG(+CAGTGT)GTACCAAAGTCCTACAGCAGAGGCTACAGCA NGG 10 8 17 8 24 0 100 5 10
    240605130208_g371 ACCCATGCTGTAGCCTCTGCTGTAGGACTTTG(+CAGTGT)GTACCCAGTTGCCATAGAGGAGTGGGGTGCC NGG 10 8 17 8 24 0 100 5 10
    240605130207_tw6q GCCACACTCCACAGAAGCCTTCTCAGGAACTG(+CAGTGT)GATCCACAACCTAGCTACAGCCCCTCGCTGA NGG 10 8 17 8 24 0 100 5 10