Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605130119_mfw8 AATGCTCAGTCTGAGTACAGGTCTCAGTCCTG(+CAGTGT)GATCCTCCATTTCTTTTTTTTAAGGGGGTGG NGG 10 8 17 8 24 0 100 5 10
    240605130118_0g77 CCTTTCTGATAGACATTATGCCCCCCCTTCAG(+CAGTGT)GTACCCGTAATTTGTCTCTGAGGGGCAGGGT NGG 10 8 17 8 24 0 100 5 10
    240605130117_nkws TTTCCACCCTGCCCCTCAGAGACAAATTACGG(+CAGTGT)GTACCTGAAGGGGGGGCATAATGTCTATCAG NGG 10 8 17 8 24 0 100 5 10
    240605130115_imt5 GTTTATGTCCAAGTGCTGGGATTACAGGTGAG(+CAGTGT)GTACCACATGTGTGGTTAAAATGGTTGGGAA NGG 10 8 17 8 24 0 100 5 10
    240605130114_i29v TTTGATTCCCAACCATTTTAACCACACATGTG(+CAGTGT)GTACCTCACCTGTAATCCCAGCACTTGGACA NGG 10 8 17 8 24 0 100 5 10
    240605130113_e3u9 AAACCCAGACTCCAGTAGCAAGCAGCTCTTAG(+CAGTGT)GATCCCTCAAGGCTGGGACTCAGATCTGGGC NGG 10 8 17 8 24 0 100 5 10
    240605130111_u6rd TTGTGGCCCAGATCTGAGTCCCAGCCTTGAGG(+CAGTGT)GATCCTAAGAGCTGCTTGCTACTGGAGTCTG NGG 10 8 17 8 24 0 100 5 10
    240605130110_fc2c AGCACATGGTGGCTCAGAACCATCTGTAATGG(+CAGTGT)GATCCGATGCCCTGTTCTGGTGTCTGAAGAT NGG 10 8 17 8 24 0 100 5 10
    240605130108_sbn3 TAGCTATCTTCAGACACCAGAACAGGGCATCG(+CAGTGT)GATCCCATTACAGATGGTTCTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240605130107_mj66 TACCCCAAAAAGGTGAGGTGTCCACAGCTGGG(+CAGTGT)GTACCACCAGTATACAGACCTTTAGAATTTG NGG 10 8 17 8 24 0 100 5 10