Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605130016_dig2 GAAACTCAGAGCCAGTGTGGCCCCGCTCCCAG(+CAGTGT)GATCCAGACCCATAGCATCCACAGGATGACA NGG 10 8 17 8 24 0 100 5 10
    240605130014_4961 GGGCAGCACGACCCATACCATGGAAAACAGGG(+CAGTGT)GATCCAGTGTGGGTTCCTCAATGGCCCCTAT NGG 10 8 17 8 24 0 100 5 10
    240605130013_qwis AGAGGATAGGGGCCATTGAGGAACCCACACTG(+CAGTGT)GATCCCCTGTTTTCCATGGTATGGGTCGTGC NGG 10 8 17 8 24 0 100 5 10
    240605130012_xpev GCAGTTTTGTGGCAGAATGGGCCATTGTTGTG(+CAGTGT)GATCCATCCAAACGTATCCCCAGCTAAAATA NGG 10 8 17 8 24 0 100 5 10
    240605130010_k2f2 TCTATTATTTTAGCTGGGGATACGTTTGGATG(+CAGTGT)GATCCACAACAATGGCCCATTCTGCCACAAA NGG 10 8 17 8 24 0 100 5 10
    240605130007_r8tp AAAATGTCCTTGATAATAAATGGTATGGCCCG(+CAGTGT)GATCCTATTTTGATAAGATCCAGGGGAGCTA NGG 10 8 17 8 24 0 100 5 10
    240605130006_lnuh ACAAATAGCTCCCCTGGATCTTATCAAAATAG(+CAGTGT)GATCCGGGCCATACCATTTATTATCAAGGAC NGG 10 8 17 8 24 0 100 5 10
    240605130004_bikd ATAGGCCTGTAACTGATCCACTAGGGCAAGGG(+CAGTGT)GTACCTCACAGACAAAAATCACATCCCTAAC NGG 10 8 17 8 24 0 100 5 10
    240605130003_1lqr CCAGAGTTAGGGATGTGATTTTTGTCTGTGAG(+CAGTGT)GTACCCCTTGCCCTAGTGGATCAGTTACAGG NGG 10 8 17 8 24 0 100 5 10
    240605130000_5oo8 CGGGATTGTTGCCTCTGTCTCCAACACCCTTG(+CAGTGT)GATCCTACCGATCCGTGGGCACACGTCCATA NGG 10 8 17 8 24 0 100 5 10