Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605125924_gjk0 AGTGCAAATTGTCTGCCCTCCTCCCCATAGGG(+CAGTGT)GATCCATCCTGAGCCCTGTGTGTACCTCCTC NGG 10 8 17 8 24 0 100 5 10
    240605125923_nbom TGGGAATCACACTTTAAAAGGGTCAGAATATG(+CAGTGT)GATCCCGAGGGAAAGAATGCTCCGCACTGTT NGG 10 8 17 8 24 0 100 5 10
    240605125920_po9p TCATGAACAGTGCGGAGCATTCTTTCCCTCGG(+CAGTGT)GATCCATATTCTGACCCTTTTAAAGTGTGAT NGG 10 8 17 8 24 0 100 5 10
    240605125919_i8ps TGCAATGCCTCAGAGACAAGAAGAGTGCATTG(+CAGTGT)GATCCTAGGGGGCTGCAGTTACAGTCTGTTG NGG 10 8 17 8 24 0 100 5 10
    240605125916_wf1y ACTCACAACAGACTGTAACTGCAGCCCCCTAG(+CAGTGT)GATCCAATGCACTCTTCTTGTCTCTGAGGCA NGG 10 8 17 8 24 0 100 5 10
    240605125914_rpas TGCAATATTTGTACACATATACAAGTCCTGAG(+CAGTGT)GATCCTAATTTCAGCTCAGAGATCGAATTGG NGG 10 8 17 8 24 0 100 5 10
    240605125913_5sss CTGACCCAATTCGATCTCTGAGCTGAAATTAG(+CAGTGT)GATCCTCAGGACTTGTATATGTGTACAAATA NGG 10 8 17 8 24 0 100 5 10
    240605125911_mjli CAAGGAGTTCTAGCATACACTTTTGTGATTGG(+CAGTGT)GTACCATATGGTTATATCTGATTCTTGGTAG NGG 10 8 17 8 24 0 100 5 10
    240605125910_teph TCATGCTACCAAGAATCAGATATAACCATATG(+CAGTGT)GTACCCAATCACAAAAGTGTATGCTAGAACT NGG 10 8 17 8 24 0 100 5 10
    240605125909_a7jl CTTAAAAGGTGGAGTTGTGGGTAGATGAAAAG(+CAGTGT)GTACCGTCCAAAAGCAGACTGTAAATTATGA NGG 10 8 17 8 24 0 100 5 10