Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605125839_azr9 AGTGTCCTTTGTAAATGTTCAAGTGAATTAAG(+CAGTGT)GTACCTGCAAGTGCAGTAACATATCTCCTGA NGG 10 8 17 8 24 0 100 5 10
    240605125837_y2bs ATAAACAACAAAGCTAGTCATTTTGTCCTATG(+CAGTGT)GATCCTGTCCTCACATTAAACACTGTCCAGT NGG 10 8 17 8 24 0 100 5 10
    240605125836_qnsg CCGCCACTGGACAGTGTTTAATGTGAGGACAG(+CAGTGT)GATCCATAGGACAAAATGACTAGCTTTGTTG NGG 10 8 17 8 24 0 100 5 10
    240605125834_sgr5 ACCCTTTTCTTAGGGCACACAAATTAATCTAG(+CAGTGT)GTACCTTGTTGTGTACAATTGAGAACACCTT NGG 10 8 17 8 24 0 100 5 10
    240605125833_627c AAGTCAAGGTGTTCTCAATTGTACACAACAAG(+CAGTGT)GTACCTAGATTAATTTGTGTGCCCTAAGAAA NGG 10 8 17 8 24 0 100 5 10
    240605125832_m13i AAAGCAAATGGCAGAGCCTTTTGATATCAGAG(+CAGTGT)GATCCTCTTACACTTCTTCCCCCCTCCATTG NGG 10 8 17 8 24 0 100 5 10
    240605125831_ue9k CAGCACAATGGAGGGGGGAAGAAGTGTAAGAG(+CAGTGT)GATCCTCTGATATCAAAAGGCTCTGCCATTT NGG 10 8 17 8 24 0 100 5 10
    240605125829_f7iv TACATAGTTCAGAGTTGCTTCTGGGGGATCTG(+CAGTGT)GTACCTTTAATATTTAAACTTTGAGCAATAT NGG 10 8 17 8 24 0 100 5 10
    240605125828_h5au GCAGAATATTGCTCAAAGTTTAAATATTAAAG(+CAGTGT)GTACCAGATCCCCCAGAAGCAACTCTGAACT NGG 10 8 17 8 24 0 100 5 10
    240605125827_id10 CCCTTGCAGATAATGTTATGCCCTTGAGATAG(+CAGTGT)GTACCCAATGCAAATTAAATGAATGTAAAAT NGG 10 8 17 8 24 0 100 5 10