Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605125811_rmrc TAGGAACTGCAAGACTGATTTCCAGAGTGATG(+CAGTGT)GTACCAGCTTGCAATCCCACCAGCAATGGAG NGG 10 8 17 8 24 0 100 5 10
    240605125809_4zvw TAACTTTATTTTAAAAAGAAAACAAAGCTTTG(+CAGTGT)GATCCCAAACCACAGATTAATGGCTACACAG NGG 10 8 17 8 24 0 100 5 10
    240605125808_091l AATCACTGTGTAGCCATTAATCTGTGGTTTGG(+CAGTGT)GATCCAAAGCTTTGTTTTCTTTTTAAAATAA NGG 10 8 17 8 24 0 100 5 10
    240605125806_ihkb TCAACTAGTCTTCCTTGTAATCAGAGAAACCG(+CAGTGT)GATCCACCAAGTCTTACTCAGCATCCAGCGA NGG 10 8 17 8 24 0 100 5 10
    240605125805_702t ACCAATCGCTGGATGCTGAGTAAGACTTGGTG(+CAGTGT)GATCCGGTTTCTCTGATTACAAGGAAGACTA NGG 10 8 17 8 24 0 100 5 10
    240605125803_vj53 AGTGTTTAACAGCACTGGCTGCTCTTCCAGAG(+CAGTGT)GTACCCAGGCTTAATTCCCAGCACCAACATG NGG 10 8 17 8 24 0 100 5 10
    240605125800_j6iz ACTGTCATGTTGGTGCTGGGAATTAAGCCTGG(+CAGTGT)GTACCTCTGGAAGAGCAGCCAGTGCTGTTAA NGG 10 8 17 8 24 0 100 5 10
    240605125759_pqgu CTAGTTTTATGTCAACATGGTACAAGCTAGAG(+CAGTGT)GTACCAGAGAGGAGGGAGCCTCAATTGGGAA NGG 10 8 17 8 24 0 100 5 10
    240605125758_9ise GCATTTTCCCAATTGAGGCTCCCTCCTCTCTG(+CAGTGT)GTACCTCTAGCTTGTACCATGTTGACATAAA NGG 10 8 17 8 24 0 100 5 10
    240605125756_d3t8 GGTGTCTTTTCACAAAACCAGCTTGCTTGGTG(+CAGTGT)GATCCTTTTGTCTTTTCCCTGGCTAGTATTT NGG 10 8 17 8 24 0 100 5 10