Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605125825_tvtp TCAGTATTTTACATTCATTTAATTTGCATTGG(+CAGTGT)GTACCTATCTCAAGGGCATAACATTATCTGC NGG 10 8 17 8 24 0 100 5 10
    240605125824_92sh GTACATAACGTCCTTCAGTCATATTCATCCAG(+CAGTGT)GTACCCTCCTTTGTCCCAGTCCCACCCCTCT NGG 10 8 17 8 24 0 100 5 10
    240605125822_6dto GGATAAGAGGGGTGGGACTGGGACAAAGGAGG(+CAGTGT)GTACCTGGATGAATATGACTGAAGGACGTTA NGG 10 8 17 8 24 0 100 5 10
    240605125821_0ct2 AGTGGCTCGTTAAGCTTTCCTTTTACCAGTTG(+CAGTGT)GTACCAACTTTTAGCATTTCATAAAGTTTGT NGG 10 8 17 8 24 0 100 5 10
    240605125819_1sj1 ATTCTACAAACTTTATGAAATGCTAAAAGTTG(+CAGTGT)GTACCAACTGGTAAAAGGAAAGCTTAACGAG NGG 10 8 17 8 24 0 100 5 10
    240605125818_1iak GTCCAGATGTGCGGCAGCCGTGGAGCTTCAGG(+CAGTGT)GTACCCTGGAGCTCACAGGCTCTCTGGGAGT NGG 10 8 17 8 24 0 100 5 10
    240605125816_iaz1 GGCAGACTCCCAGAGAGCCTGTGAGCTCCAGG(+CAGTGT)GTACCCTGAAGCTCCACGGCTGCCGCACATC NGG 10 8 17 8 24 0 100 5 10
    240605125814_ye2j TACATTAGCAGGGGAGGCCTGCAGTAACAGAG(+CAGTGT)GATCCTAAAGAATGAACTGGCCGGACTCACT NGG 10 8 17 8 24 0 100 5 10
    240605125813_nrin GGAAGAGTGAGTCCGGCCAGTTCATTCTTTAG(+CAGTGT)GATCCTCTGTTACTGCAGGCCTCCCCTGCTA NGG 10 8 17 8 24 0 100 5 10
    240605125812_jnrw CACTCCTCCATTGCTGGTGGGATTGCAAGCTG(+CAGTGT)GTACCATCACTCTGGAAATCAGTCTTGCAGT NGG 10 8 17 8 24 0 100 5 10