Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605125907_sspf TGCTGTCATAATTTACAGTCTGCTTTTGGACG(+CAGTGT)GTACCTTTTCATCTACCCACAACTCCACCTT NGG 10 8 17 8 24 0 100 5 10
    240605125906_4u2h TGCCAGTGAATTCTCTGAGGAAGCCTGCGATG(+CAGTGT)GATCCTTTCAGGTGACTTATTACATAGGGTT NGG 10 8 17 8 24 0 100 5 10
    240605125905_c5b4 GAGAGAACCCTATGTAATAAGTCACCTGAAAG(+CAGTGT)GATCCATCGCAGGCTTCCTCAGAGAATTCAC NGG 10 8 17 8 24 0 100 5 10
    240605125904_22rw GAAAGAGTTGGCAATTCAGACTTTCTTGTTTG(+CAGTGT)GATCCTATTATCAGCTCCTTAGGGTTTGTAT NGG 10 8 17 8 24 0 100 5 10
    240605125902_9sh5 TCAAGATACAAACCCTAAGGAGCTGATAATAG(+CAGTGT)GATCCAAACAAGAAAGTCTGAATTGCCAACT NGG 10 8 17 8 24 0 100 5 10
    240605125901_kqb7 CCTGCAGTGTATACAAAAGAATTTACTCCTTG(+CAGTGT)GATCCCTTGTGTTTGGCTACAGTTACCTGTT NGG 10 8 17 8 24 0 100 5 10
    240605125859_kgy1 TTCCAAACAGGTAACTGTAGCCAAACACAAGG(+CAGTGT)GATCCAAGGAGTAAATTCTTTTGTATACACT NGG 10 8 17 8 24 0 100 5 10
    240605125857_1mgk GGTGCCAGCCTTGGGGCACACCGCTCCACCGG(+CAGTGT)GTACCGCTGCAGCCACTGTGTTACTGTAAGC NGG 10 8 17 8 24 0 100 5 10
    240605125855_qu2h GTGTGGCTTACAGTAACACAGTGGCTGCAGCG(+CAGTGT)GTACCCGGTGGAGCGGTGTGCCCCAAGGCTG NGG 10 8 17 8 24 0 100 5 10
    240605125854_ee8p ATATCATAGACTATAATAGAAAACAACATTTG(+CAGTGT)GATCCATTGGAAGTGCAAAGATAAAGTATTT NGG 10 8 17 8 24 0 100 5 10