Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605125959_0tnc AGGATTATGGACGTGTGCCCACGGATCGGTAG(+CAGTGT)GATCCAAGGGTGTTGGAGACAGAGGCAACAA NGG 10 8 17 8 24 0 100 5 10
    240605125957_kc1z TCCCCAGTTGCCAACTCACTGCAGGTGACACG(+CAGTGT)GTACCTCAGGAAGTGTAAGGACCCTTCTCCT NGG 10 8 17 8 24 0 100 5 10
    240605125955_h27h GGTGGAGGAGAAGGGTCCTTACACTTCCTGAG(+CAGTGT)GTACCGTGTCACCTGCAGTGAGTTGGCAACT NGG 10 8 17 8 24 0 100 5 10
    240605125954_gb24 GTCAGCTCTTTCCTTTCACATGTGTGATTCAG(+CAGTGT)GTACCTTTACCTGCTGAGCAAGTCCTAAACT NGG 10 8 17 8 24 0 100 5 10
    240605125953_ygy0 TAAGAAGTTTAGGACTTGCTCAGCAGGTAAAG(+CAGTGT)GTACCTGAATCACACATGTGAAAGGAAAGAG NGG 10 8 17 8 24 0 100 5 10
    240605125951_erdi TATTCATTTGGAGGTTCTTAATTTGAAGATGG(+CAGTGT)GATCCAGGTTTGGACTGACTGGAGATATTTG NGG 10 8 17 8 24 0 100 5 10
    240605125949_qk47 ATCACCAAATATCTCCAGTCAGTCCAAACCTG(+CAGTGT)GATCCCATCTTCAAATTAAGAACCTCCAAAT NGG 10 8 17 8 24 0 100 5 10
    240605125948_ahix TCCTTATAGCACTCAAGTGGCAGAGGCAGAAG(+CAGTGT)GATCCCTAAGAATTTGAACCAGACTAGGTTA NGG 10 8 17 8 24 0 100 5 10
    240605125946_um25 CTATGTAACCTAGTCTGGTTCAAATTCTTAGG(+CAGTGT)GATCCTTCTGCCTCTGCCACTTGAGTGCTAT NGG 10 8 17 8 24 0 100 5 10
    240605125945_ltuc AAGTCCTCACAGAGGACACAGCTCAGGATCGG(+CAGTGT)GATCCCACACCTGTAAAAAGTAAAGAACAAG NGG 10 8 17 8 24 0 100 5 10