Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605130033_qlax GTTTGCTGAGTGGGTGCAGCTTTCTCCAGTGG(+CAGTGT)GTACCTGGGTCAATGTCCAGTAGGCCCCTGA NGG 10 8 17 8 24 0 100 5 10
    240605130031_zek5 CTGGGATGGGAGCCACCTTGTTTGGTGATCAG(+CAGTGT)GATCCTGGTGAACTTGTTAGTAAGTGCTCAG NGG 10 8 17 8 24 0 100 5 10
    240605130028_axsn GGCCCCTGAGCACTTACTAACAAGTTCACCAG(+CAGTGT)GATCCTGATCACCAAACAAGGTGGCTCCCAT NGG 10 8 17 8 24 0 100 5 10
    240605130027_eyds CTGGGTCTTGGGAGCTCTTGCCTTCACCTGTG(+CAGTGT)GATCCACTTAAGACATTGTGGTCTGAAGCTC NGG 10 8 17 8 24 0 100 5 10
    240605130025_qckz GGCCTGAGCTTCAGACCACAATGTCTTAAGTG(+CAGTGT)GATCCACAGGTGAAGGCAAGAGCTCCCAAGA NGG 10 8 17 8 24 0 100 5 10
    240605130024_5rio GATCTTGCCTGTGAGATCCAGAAGAAGTGTTG(+CAGTGT)GATCCTCTGGAGCTGGAGTTAGAGGTAGCTG NGG 10 8 17 8 24 0 100 5 10
    240605130022_sc27 TCTCTCAGCTACCTCTAACTCCAGCTCCAGAG(+CAGTGT)GATCCAACACTTCTTCTGGATCTCACAGGCA NGG 10 8 17 8 24 0 100 5 10
    240605130021_29tg CCGACATTCCAAGGAGCTATGGTTGTCACATG(+CAGTGT)GTACCAGCAGCTGGAAGACGGGACTAACGTA NGG 10 8 17 8 24 0 100 5 10
    240605130020_8l8l AATGATACGTTAGTCCCGTCTTCCAGCTGCTG(+CAGTGT)GTACCATGTGACAACCATAGCTCCTTGGAAT NGG 10 8 17 8 24 0 100 5 10
    240605130019_50zp AAGTCTGTCATCCTGTGGATGCTATGGGTCTG(+CAGTGT)GATCCTGGGAGCGGGGCCACACTGGCTCTGA NGG 10 8 17 8 24 0 100 5 10