Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605130049_7wuz GGATCCAACCCAGGTCACAGGCCTGGGACTTG(+CAGTGT)GTACCCTGGTTGGACACTCCTGAGACTCCAA NGG 10 8 17 8 24 0 100 5 10
    240605130048_i4hj ACTTGGGGTAAGAAGGGCATCGGTCTGTGAAG(+CAGTGT)GTACCCCTTCTCTCTCAAAAATGGGGATAAG NGG 10 8 17 8 24 0 100 5 10
    240605130046_cg7d TCAAGCTTATCCCCATTTTTGAGAGAGAAGGG(+CAGTGT)GTACCTTCACAGACCGATGCCCTTCTTACCC NGG 10 8 17 8 24 0 100 5 10
    240605130045_4xhq TCACTGACAGGAGAGCAGCCAGGGGAGGCTAG(+CAGTGT)GTACCTGGGGCTGCTGCGGCTGCCAGAAACC NGG 10 8 17 8 24 0 100 5 10
    240605130042_o5nu GAGATGGTTTCTGGCAGCCGCAGCAGCCCCAG(+CAGTGT)GTACCTAGCCTCCCCTGGCTGCTCTCCTGTC NGG 10 8 17 8 24 0 100 5 10
    240605130041_eq1f GGTACCAAGTCCCAGGCCTGTGACCTGGGTTG(+CAGTGT)GATCCATGCTATGGGCAGAAGGGGGCTTTGG NGG 10 8 17 8 24 0 100 5 10
    240605130039_typr CTTTCCCAAAGCCCCCTTCTGCCCATAGCATG(+CAGTGT)GATCCAACCCAGGTCACAGGCCTGGGACTTG NGG 10 8 17 8 24 0 100 5 10
    240605130038_4t9t TTTTGGTTGTTTTGTTTAAAAAGACATTTGAG(+CAGTGT)GATCCAGAGAGATGGGTCAGTGGGTAAAGTC NGG 10 8 17 8 24 0 100 5 10
    240605130037_q7vd CAAGTGACTTTACCCACTGACCCATCTCTCTG(+CAGTGT)GATCCTCAAATGTCTTTTTAAACAAAACAAC NGG 10 8 17 8 24 0 100 5 10
    240605130034_hxb3 AGTGCTCAGGGGCCTACTGGACATTGACCCAG(+CAGTGT)GTACCCACTGGAGAAAGCTGCACCCACTCAG NGG 10 8 17 8 24 0 100 5 10