Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605130607_asmg ATCTAAATTCATAAATGGTATGAGGCACTTGG(+CAGTGT)GTACCCCTGTGATGAAACTTGTGTGCTTCCT NGG 10 8 17 8 24 0 100 5 10
    240605130605_wtv0 CTGCTAGGAAGCACACAAGTTTCATCACAGGG(+CAGTGT)GTACCCAAGTGCCTCATACCATTTATGAATT NGG 10 8 17 8 24 0 100 5 10
    240605130603_jyqh ATCTAAATTCATAAATGGTATGAGGCACTTGG(+CAGTGT)GTACCCCTGTGATGAAACTTGTGTGCTTCCT NGG 10 8 17 8 24 0 100 5 10
    240605130602_fyi8 CAGGTCCTGTAGTTGTCTTCTTCTCATGTTTG(+CAGTGT)GTACCGCTAAGCTTTTCAAACTGTCTTTTCA NGG 10 8 17 8 24 0 100 5 10
    240605130601_cot3 AGGGTTGAAAAGACAGTTTGAAAAGCTTAGCG(+CAGTGT)GTACCAAACATGAGAAGAAGACAACTACAGG NGG 10 8 17 8 24 0 100 5 10
    240605130600_wve4 TTGTGTGTATTTATTAGAGCATTTGCATACTG(+CAGTGT)GTACCTTTTATTAAAAGGGTTGATTTGGTGT NGG 10 8 17 8 24 0 100 5 10
    240605130558_tyzs GTAAAACACCAAATCAACCCTTTTAATAAAAG(+CAGTGT)GTACCAGTATGCAAATGCTCTAATAAATACA NGG 10 8 17 8 24 0 100 5 10
    240605130557_hye7 TGATTCTTTAACCGTGGGACCACCTGAAAAGG(+CAGTGT)GATCCTCCTCTGCAGAGCTGTGCCTGTCTTA NGG 10 8 17 8 24 0 100 5 10
    240605130555_yda0 ACTGCTAAGACAGGCACAGCTCTGCAGAGGAG(+CAGTGT)GATCCCTTTTCAGGTGGTCCCACGGTTAAAG NGG 10 8 17 8 24 0 100 5 10
    240605130554_jr4i TCTTGTAGCCATCACCTGCCTGGGCAGGCACG(+CAGTGT)GATCCCTCTATACCATTTACCCAGCAAACCA NGG 10 8 17 8 24 0 100 5 10