Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605130440_99uo AACTGTGGCCTACTTTCTGTGGATGGAAGGAG(+CAGTGT)GTACCTAAAAAGTGTGGAGTGCATTTGGTAT NGG 10 8 17 8 24 0 100 5 10
    240605130439_h0op CTCACCTTCCTGCTGGAGGCTTTGACTGCTGG(+CAGTGT)GTACCATGAGAAACACTGAGTATCAGTCAGT NGG 10 8 17 8 24 0 100 5 10
    240605130437_1y4l CCTACACTGACTGATACTCAGTGTTTCTCATG(+CAGTGT)GTACCCAGCAGTCAAAGCCTCCAGCAGGAAG NGG 10 8 17 8 24 0 100 5 10
    240605130436_96c7 ATAGAGAAATAAATATAGTGTGGAGTTTATGG(+CAGTGT)GTACCCCTCAGTCTGCTAATCTTAGTGTTCA NGG 10 8 17 8 24 0 100 5 10
    240605130434_2fud GATGTTGAACACTAAGATTAGCAGACTGAGGG(+CAGTGT)GTACCCATAAACTCCACACTATATTTATTTC NGG 10 8 17 8 24 0 100 5 10
    240605130433_qn9z GTTTCTGTGTGCTAAGAGTCCAAGTCCACACG(+CAGTGT)GTACCTCTGACTCCGTACTTGTTCCATTCAT NGG 10 8 17 8 24 0 100 5 10
    240605130431_9p2s TTAAAATGAATGGAACAAGTACGGAGTCAGAG(+CAGTGT)GTACCGTGTGGACTTGGACTCTTAGCACACA NGG 10 8 17 8 24 0 100 5 10
    240605130430_ones CCCATGCCAGAGAGCCATGCTTTCTGAGTCTG(+CAGTGT)GTACCAACCTGAACGGTGGGACATTCTTCCT NGG 10 8 17 8 24 0 100 5 10
    240605130429_lo9q GAACCAGGAAGAATGTCCCACCGTTCAGGTTG(+CAGTGT)GTACCAGACTCAGAAAGCATGGCTCTCTGGC NGG 10 8 17 8 24 0 100 5 10
    240605130427_t1z4 TTAAAATAGAGAAATTGACAGCATTCACCATG(+CAGTGT)GTACCCAGGGGTTAGAGCTGGTGTCAAGAGT NGG 10 8 17 8 24 0 100 5 10