Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605122343_7gfb CAACAGATGCTTTTGGCAATACATTAGAAGAG(+CAGTGT)GTACCTACTCTGACAGTCGACGATCAGCTAA NGG 10 8 17 8 24 0 100 5 10
    240605122341_g4su CAGATAATTACTATCCCGGAAAACCTAGAGAG(+CAGTGT)GTACCCTGAGCCAGAACCCCTCAGCTGAGCA NGG 10 8 17 8 24 0 100 5 10
    240605122340_0wp9 TCTGTTGCTCAGCTGAGGGGTTCTGGCTCAGG(+CAGTGT)GTACCTCTCTAGGTTTTCCGGGATAGTAATT NGG 10 8 17 8 24 0 100 5 10
    240605122338_pr7l TAGGCCTGTGCCATACCAAATATTCACCAATG(+CAGTGT)GTACCCTCTAGAGTAAAGCAAAGCTAATAAA NGG 10 8 17 8 24 0 100 5 10
    240605122336_9jg0 CAACTTTTATTAGCTTTGCTTTACTCTAGAGG(+CAGTGT)GTACCATTGGTGAATATTTGGTATGGCACAG NGG 10 8 17 8 24 0 100 5 10
    240605122335_o1ga TTCCAGCCTGCGGGTCTTGGGCACTGAACTTG(+CAGTGT)GATCCGCAGGCTTGGCAGCAGGAGCAGGTAC NGG 10 8 17 8 24 0 100 5 10
    240605122333_f35a AGCTTGTACCTGCTCCTGCTGCCAAGCCTGCG(+CAGTGT)GATCCAAGTTCAGTGCCCAAGACCCGCAGGC NGG 10 8 17 8 24 0 100 5 10
    240605122332_y9ow TTTTAACCAAACAGATATTAAAATACAGTCTG(+CAGTGT)GATCCAGGCCAACAACACATCAAGCAGACTG NGG 10 8 17 8 24 0 100 5 10
    240605122330_64xo TTCTTCAGTCTGCTTGATGTGTTGTTGGCCTG(+CAGTGT)GATCCAGACTGTATTTTAATATCTGTTTGGT NGG 10 8 17 8 24 0 100 5 10
    240605122329_tpod GTTACATATGCATGCAAAGAAATTAACTCAAG(+CAGTGT)GTACCCTGGTGTGTTGGCTTTTCAAACTTGA NGG 10 8 17 8 24 0 100 5 10