Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605122242_5pp6 CCTGGTGCCCTTGGAGGCAGTAAGAGGGCATG(+CAGTGT)GATCCCCTGGAACTGGAGTTACAGACAGTTG NGG 10 8 17 8 24 0 100 5 10
    240605122239_6swg GTTCACAACTGTCTGTAACTCCAGTTCCAGGG(+CAGTGT)GATCCATGCCCTCTTACTGCCTCCAAGGGCA NGG 10 8 17 8 24 0 100 5 10
    240605122238_z1zs CTATCTTCAGACACAACCAGAGGAGGGCATTG(+CAGTGT)GATCCTATTACCAATGGTTGTAAGCTACCAT NGG 10 8 17 8 24 0 100 5 10
    240605122237_3k2a ACCACATGGTAGCTTACAACCATTGGTAATAG(+CAGTGT)GATCCAATGCCCTCCTCTGGTTGTGTCTGAA NGG 10 8 17 8 24 0 100 5 10
    240605122235_hzcd CCCACTCCCACTCCCACTCCCAACCCCCATTG(+CAGTGT)GATCCCTGGATTGTCAGTCAAGAATGAAAAA NGG 10 8 17 8 24 0 100 5 10
    240605122233_imjm TGGGGTTTTTCATTCTTGACTGACAATCCAGG(+CAGTGT)GATCCAATGGGGGTTGGGAGTGGGAGTGGGA NGG 10 8 17 8 24 0 100 5 10
    240605122232_orqy CCATTCATAGTTCCATAACTCCGGTTCTGTAG(+CAGTGT)GATCCGAGGTTCTTTTCTCACCCGTACGAAA NGG 10 8 17 8 24 0 100 5 10
    240605122230_o474 ATGCATTTCGTACGGGTGAGAAAAGAACCTCG(+CAGTGT)GATCCTACAGAACCGGAGTTATGGAACTATG NGG 10 8 17 8 24 0 100 5 10
    240605122228_1d36 TCCTCAGAAAATTGGACATAGTACTACCGGAG(+CAGTGT)GATCCAGCAATACCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10
    240605122227_c61t TTCTGGATATATGCCCAGGAGAGGTATTGCTG(+CAGTGT)GATCCTCCGGTAGTACTATGTCCAATTTTCT NGG 10 8 17 8 24 0 100 5 10