Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605122139_qoed GCACTGCCAGACTTACTTACTAAGCCTGCTGG(+CAGTGT)GATCCCAGGCCTGCCCTGGGACAGAGAGGCC NGG 10 8 17 8 24 0 100 5 10
    240605122137_udpf TTCTCGGCCTCTCTGTCCCAGGGCAGGCCTGG(+CAGTGT)GATCCCAGCAGGCTTAGTAAGTAAGTCTGGC NGG 10 8 17 8 24 0 100 5 10
    240605122135_ou12 AAAACTGTAGCTTACTCATCTTAGTAGGTTAG(+CAGTGT)GTACCAAAGTCCAGGCTGTTTATTTCTAACC NGG 10 8 17 8 24 0 100 5 10
    240605122134_jsg6 ATTTGGGTTAGAAATAAACAGCCTGGACTTTG(+CAGTGT)GTACCTAACCTACTAAGATGAGTAAGCTACA NGG 10 8 17 8 24 0 100 5 10
    240605122133_9i99 TGTCATCCCTCTTTGGGCCCGAAGGCCAGAGG(+CAGTGT)GTACCAACTGGGCAAATGCCATCTGCCTTTT NGG 10 8 17 8 24 0 100 5 10
    240605122131_m7sr TGGGTAAAAGGCAGATGGCATTTGCCCAGTTG(+CAGTGT)GTACCCTCTGGCCTTCGGGCCCAAAGAGGGA NGG 10 8 17 8 24 0 100 5 10
    240605122130_5vxa CCTAGGTTATGAATGACAGCCACAGATGCTGG(+CAGTGT)GATCCCTATTGAGAAGGCAGTGCTATCGAGA NGG 10 8 17 8 24 0 100 5 10
    240605122128_vxbg CGGGCTCTCGATAGCACTGCCTTCTCAATAGG(+CAGTGT)GATCCCAGCATCTGTGGCTGTCATTCATAAC NGG 10 8 17 8 24 0 100 5 10
    240605122126_s65p GGTTGCTCCGTGCATAACTAGTTCTTTCTGGG(+CAGTGT)GATCCTGTCTGTCTCTTTTTTCCCCTGACCT NGG 10 8 17 8 24 0 100 5 10
    240605122124_ztc4 GACAGAGGTCAGGGGAAAAAAGAGACAGACAG(+CAGTGT)GATCCCCAGAAAGAACTAGTTATGCACGGAG NGG 10 8 17 8 24 0 100 5 10