Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605122053_yj0y GCAAAAGTGTCTTCCCCCTCCCCCGACTTCAG(+CAGTGT)GATCCTGAAGGAAGGACTGGGTGCGGTGCGG NGG 10 8 17 8 24 0 100 5 10
    240605122052_329g TCTTGCCGCACCGCACCCAGTCCTTCCTTCAG(+CAGTGT)GATCCTGAAGTCGGGGGAGGGGGAAGACACT NGG 10 8 17 8 24 0 100 5 10
    240605122051_h07x AGCTGGTGCCCCCTCCCCAAAGACAACTGTAG(+CAGTGT)GTACCATCTAGTGAACAGCAAGACCTAGACT NGG 10 8 17 8 24 0 100 5 10
    240605122049_q6gu ATTCCAGTCTAGGTCTTGCTGTTCACTAGATG(+CAGTGT)GTACCTACAGTTGTCTTTGGGGAGGGGGCAC NGG 10 8 17 8 24 0 100 5 10
    240605122048_3hxq GCTAGGCAAGACAGAATGGAGGGCATATTTAG(+CAGTGT)GATCCCACCCAGCAGTCTACAGCTCATCTCC NGG 10 8 17 8 24 0 100 5 10
    240605122046_076y TCTTTGGAGATGAGCTGTAGACTGCTGGGTGG(+CAGTGT)GATCCTAAATATGCCCTCCATTCTGTCTTGC NGG 10 8 17 8 24 0 100 5 10
    240605122045_ikir AGTAGACTAGGTTAGCTGAGGAGTGTGCAAGG(+CAGTGT)GATCCCTTACCTCTCCTTCCCTGCTTTCTGC NGG 10 8 17 8 24 0 100 5 10
    240605122043_8553 AGAGAGCAGAAAGCAGGGAAGGAGAGGTAAGG(+CAGTGT)GATCCCTTGCACACTCCTCAGCTAACCTAGT NGG 10 8 17 8 24 0 100 5 10
    240605122041_yhal AGCTAAGTCTATAGATCCAACTTTCTGACCTG(+CAGTGT)GATCCAGACAACACTACTTTGGAAAGAGTGT NGG 10 8 17 8 24 0 100 5 10
    240605122040_o5a4 TATTAACACTCTTTCCAAAGTAGTGTTGTCTG(+CAGTGT)GATCCAGGTCAGAAAGTTGGATCTATAGACT NGG 10 8 17 8 24 0 100 5 10