Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605121951_abyf TGACAGGCAATTTCAACAGTTTAAAGTGTTTG(+CAGTGT)GTACCGTGGCTTCTGAGCGACCTGCAGTGGG NGG 10 8 17 8 24 0 100 5 10
    240605121949_v2v6 CCACACCCACTGCAGGTCGCTCAGAAGCCACG(+CAGTGT)GTACCAAACACTTTAAACTGTTGAAATTGCC NGG 10 8 17 8 24 0 100 5 10
    240605121948_2vx6 CTATCAGGAGCTTCAGCCAGCCCCCTACATAG(+CAGTGT)GTACCTGCCAGGAGGCAGAGGGGCAGGCCAG NGG 10 8 17 8 24 0 100 5 10
    240605121946_wumc GTCCTCTGGCCTGCCCCTCTGCCTCCTGGCAG(+CAGTGT)GTACCTATGTAGGGGGCTGGCTGAAGCTCCT NGG 10 8 17 8 24 0 100 5 10
    240605121945_q6by CTCAAGGCCGGTGTGCTGTAAGTTAGGGTTTG(+CAGTGT)GATCCTCTCCCTCTCATTTGTATATTGTTTC NGG 10 8 17 8 24 0 100 5 10
    240605121944_fe1a TCGAGGAAACAATATACAAATGAGAGGGAGAG(+CAGTGT)GATCCAAACCCTAACTTACAGCACACCGGCC NGG 10 8 17 8 24 0 100 5 10
    240605121943_5lkv AGAGACGGTGGTCTCAACTACATGCACAAGTG(+CAGTGT)GATCCTAAAAAATGTATGGAGATTTTTTAAA NGG 10 8 17 8 24 0 100 5 10
    240605121942_8677 TTCCATTTAAAAAATCTCCATACATTTTTTAG(+CAGTGT)GATCCACTTGTGCATGTAGTTGAGACCACCG NGG 10 8 17 8 24 0 100 5 10
    240605121940_5uqa TGGCTACTTTACGTGTACGTGAATGAGGCTGG(+CAGTGT)GTACCAGATGTAAAGGCTAAGGGAGAGGAGA NGG 10 8 17 8 24 0 100 5 10
    240605121938_nn5q TGTAGTCTCCTCTCCCTTAGCCTTTACATCTG(+CAGTGT)GTACCCAGCCTCATTCACGTACACGTAAAGT NGG 10 8 17 8 24 0 100 5 10