Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605122038_194h AAGGAGTTTTACTCCTTGGTTTCAAAGCAGAG(+CAGTGT)GATCCGCCTATGGACAGGTTCATGAAAATGA NGG 10 8 17 8 24 0 100 5 10
    240605122036_q0lu ATCCATCATTTTCATGAACCTGTCCATAGGCG(+CAGTGT)GATCCTCTGCTTTGAAACCAAGGAGTAAAAC NGG 10 8 17 8 24 0 100 5 10
    240605122034_rkza GGTATCATCTCCAGTTTCAGGCATCCAGTGGG(+CAGTGT)GATCCCTGACAGGTGGGAGAGAACTACTATA NGG 10 8 17 8 24 0 100 5 10
    240605122032_yahn ATTATTATAGTAGTTCTCTCCCACCTGTCAGG(+CAGTGT)GATCCCCACTGGATGCCTGAAACTGGAGATG NGG 10 8 17 8 24 0 100 5 10
    240605122031_ymoa AAAGGATAACTTTTCTTTCTTTTGACTATGTG(+CAGTGT)GATCCTACATATTGAACTCAGATAGTCAGGG NGG 10 8 17 8 24 0 100 5 10
    240605122029_z8ok GCTAACCCTGACTATCTGAGTTCAATATGTAG(+CAGTGT)GATCCACATAGTCAAAAGAAAGAAAAGTTAT NGG 10 8 17 8 24 0 100 5 10
    240605122028_8ql6 GGGGGGGGGCGGGGGTGGTGTGGTCTGAACAG(+CAGTGT)GATCCCACAGTAGCTCAGGCTGGCCTTGAAC NGG 10 8 17 8 24 0 100 5 10
    240605122026_ig2i AGAAAGTTCAAGGCCAGCCTGAGCTACTGTGG(+CAGTGT)GATCCTGTTCAGACCACACCACCCCCGCCCC NGG 10 8 17 8 24 0 100 5 10
    240605122025_m8bc ATGGAATCTTATTCCAACACAACTTCCAAATG(+CAGTGT)GATCCTCTCCTCAAAATAATCTGGTATGAAG NGG 10 8 17 8 24 0 100 5 10
    240605122024_vawr AGTTTCTTCATACCAGATTATTTTGAGGAGAG(+CAGTGT)GATCCATTTGGAAGTTGTGTTGGAATAAGAT NGG 10 8 17 8 24 0 100 5 10