Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605122022_mv9t AATACATTCAGACTTCAGCTCTCCAGAGGAAG(+CAGTGT)GTACCCAGATCAATTTTTAGGTGGGAAAAAT NGG 10 8 17 8 24 0 100 5 10
    240605122021_kymh CTGTCATTTTTCCCACCTAAAAATTGATCTGG(+CAGTGT)GTACCTTCCTCTGGAGAGCTGAAGTCTGAAT NGG 10 8 17 8 24 0 100 5 10
    240605122019_1tj8 CAATTCGCTCCTTCTGTTCCCCACATTCCAGG(+CAGTGT)GATCCTTCGGAGCAGGTTCTGTACGTCCAGC NGG 10 8 17 8 24 0 100 5 10
    240605122017_i263 GTGGGGCTGGACGTACAGAACCTGCTCCGAAG(+CAGTGT)GATCCCTGGAATGTGGGGAACAGAAGGAGCG NGG 10 8 17 8 24 0 100 5 10
    240605122016_y6yb TTCTTCCACCCTTCTAGCCACTCTCTGGTTTG(+CAGTGT)GTACCTCCTCAAAACATCTGGGCTCCTCTGC NGG 10 8 17 8 24 0 100 5 10
    240605122014_o67q TGTATGCAGAGGAGCCCAGATGTTTTGAGGAG(+CAGTGT)GTACCAAACCAGAGAGTGGCTAGAAGGGTGG NGG 10 8 17 8 24 0 100 5 10
    240605122011_z4ty GGCTGTGTCCACACCAGCAGCAGGATCCTTGG(+CAGTGT)GTACCCTTTGCTGCTTCCTTTCCCTTCCAGG NGG 10 8 17 8 24 0 100 5 10
    240605122010_c3as TTGTGCCTGGAAGGGAAAGGAAGCAGCAAAGG(+CAGTGT)GTACCCAAGGATCCTGCTGCTGGTGTGGACA NGG 10 8 17 8 24 0 100 5 10
    240605122008_joyo GGGAAACTATTACTTCAAATGAACAGTATTGG(+CAGTGT)GATCCTTCTGCCTTACTTAGTTACCTAGTAT NGG 10 8 17 8 24 0 100 5 10
    240605122006_ldsx TTCAGATACTAGGTAACTAAGTAAGGCAGAAG(+CAGTGT)GATCCCAATACTGTTCATTTGAAGTAATAGT NGG 10 8 17 8 24 0 100 5 10