Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605122109_unbk AGCCGTGGAACACGTCTCTTAGGGTGTGCTTG(+CAGTGT)GATCCAGAGCCCTGCAGGTCTCTGGGGTTTG NGG 10 8 17 8 24 0 100 5 10
    240605122107_2q1w AGTGACAAACCCCAGAGACCTGCAGGGCTCTG(+CAGTGT)GATCCAAGCACACCCTAAGAGACGTGTTCCA NGG 10 8 17 8 24 0 100 5 10
    240605122106_zwo8 TCTCTCTATCCCTTAAAAAGAACTATCTCCAG(+CAGTGT)GTACCCAGTCCTGTTGTAGAAACCACCTGAT NGG 10 8 17 8 24 0 100 5 10
    240605122104_j24n TGGGCATCAGGTGGTTTCTACAACAGGACTGG(+CAGTGT)GTACCTGGAGATAGTTCTTTTTAAGGGATAG NGG 10 8 17 8 24 0 100 5 10
    240605122102_idkk TTGTTCTCCAGACCTGACCCAGGTAGGCAAGG(+CAGTGT)GTACCCTCCCAGTCTCACCTACCATACTGTG NGG 10 8 17 8 24 0 100 5 10
    240605122100_5mfr GATGGCACAGTATGGTAGGTGAGACTGGGAGG(+CAGTGT)GTACCCTTGCCTACCTGGGTCAGGTCTGGAG NGG 10 8 17 8 24 0 100 5 10
    240605122059_qebx TGCTAAACCGTTAAGTAGCTGGAAGATATCTG(+CAGTGT)GATCCTTCTATGCCCTCTCACCCGTAGGCCC NGG 10 8 17 8 24 0 100 5 10
    240605122058_q6nu GAAGGGGGCCTACGGGTGAGAGGGCATAGAAG(+CAGTGT)GATCCAGATATCTTCCAGCTACTTAACGGTT NGG 10 8 17 8 24 0 100 5 10
    240605122056_wdly TTACAGTGACCCCCTTTGTTTGCAGTGAAGGG(+CAGTGT)GATCCTTTGCTTCTCGGTCCTCAACACGATG NGG 10 8 17 8 24 0 100 5 10
    240605122054_6pja ACCCTCATCGTGTTGAGGACCGAGAAGCAAAG(+CAGTGT)GATCCCCTTCACTGCAAACAAAGGGGGTCAC NGG 10 8 17 8 24 0 100 5 10