Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605122225_qwq1 GGATGGACCATGTAGAGACTGCCATATCCAGG(+CAGTGT)GATCCACCCCATAATCAGCATCCAAACGCTG NGG 10 8 17 8 24 0 100 5 10
    240605122223_2qkf GGTGTCAGCGTTTGGATGCTGATTATGGGGTG(+CAGTGT)GATCCCTGGATATGGCAGTCTCTACATGGTC NGG 10 8 17 8 24 0 100 5 10
    240605122221_amju CTGTGCTGCCAGCCCCTGCCCAGGTTCCTTTG(+CAGTGT)GATCCGCAGATGAAAGACACACACACACACA NGG 10 8 17 8 24 0 100 5 10
    240605122220_5588 GTGTGTGTGTGTGTGTGTGTCTTTCATCTGCG(+CAGTGT)GATCCAAAGGAACCTGGGCAGGGGCTGGCAG NGG 10 8 17 8 24 0 100 5 10
    240605122219_jiyb TTAATTTCACAGATTCTTGTTCCAATGTCTAG(+CAGTGT)GATCCATACATGATCTTTAGGGCTCCTTCAA NGG 10 8 17 8 24 0 100 5 10
    240605122218_xav1 GCCCTTTGAAGGAGCCCTAAAGATCATGTATG(+CAGTGT)GATCCTAGACATTGGAACAAGAATCTGTGAA NGG 10 8 17 8 24 0 100 5 10
    240605122216_bjnj TTTAGATGATAAATTTCTCATGTCAATTTTTG(+CAGTGT)GTACCATTATTATCTATATTTAATAGATAAG NGG 10 8 17 8 24 0 100 5 10
    240605122215_lz2r TTTCTCTTATCTATTAAATATAGATAATAATG(+CAGTGT)GTACCAAAAATTGACATGAGAAATTTATCAT NGG 10 8 17 8 24 0 100 5 10
    240605122214_zno9 CTAAATTTGTAAATGGCAGCAGTATTATAAAG(+CAGTGT)GATCCAAATGACCTAACAGGAACCAGAAAAA NGG 10 8 17 8 24 0 100 5 10
    240605122212_975b CAATTTTTTTCTGGTTCCTGTTAGGTCATTTG(+CAGTGT)GATCCTTTATAATACTGCTGCCATTTACAAA NGG 10 8 17 8 24 0 100 5 10