Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605122327_zx16 CCATTTCAAGTTTGAAAAGCCAACACACCAGG(+CAGTGT)GTACCTTGAGTTAATTTCTTTGCATGCATAT NGG 10 8 17 8 24 0 100 5 10
    240605122326_473w TACTGGGGCTGGTGAAATGAGGTGAATGACAG(+CAGTGT)GATCCGTTGGTAAAACAGCCTGGTTTTGATT NGG 10 8 17 8 24 0 100 5 10
    240605122324_lnbt CAGGAAATCAAAACCAGGCTGTTTTACCAACG(+CAGTGT)GATCCTGTCATTCACCTCATTTCACCAGCCC NGG 10 8 17 8 24 0 100 5 10
    240605122322_hgni ATAATTACAAAAAATTTGTTTACATTCCACAG(+CAGTGT)GTACCTTAATTTAAAATAAATCAGACTAACA NGG 10 8 17 8 24 0 100 5 10
    240605122321_t9ev CCACCTGTTAGTCTGATTTATTTTAAATTAAG(+CAGTGT)GTACCTGTGGAATGTAAACAAATTTTTTGTA NGG 10 8 17 8 24 0 100 5 10
    240605122319_jrnk GACCTGAATTCTCCACACAGTTGATCCTGCTG(+CAGTGT)GATCCCACACATGAGAATCATATAAGAACTG NGG 10 8 17 8 24 0 100 5 10
    240605122318_dwzc CAGGACAGTTCTTATATGATTCTCATGTGTGG(+CAGTGT)GATCCAGCAGGATCAACTGTGTGGAGAATTC NGG 10 8 17 8 24 0 100 5 10
    240605122316_iale TGTCCTTCCCACAGCCTCCTGGGCTGGTGCCG(+CAGTGT)GATCCGGGAAGGATGAGTTATCATCTAACCT NGG 10 8 17 8 24 0 100 5 10
    240605122313_4ygf GCTGTAGGTTAGATGATAACTCATCCTTCCCG(+CAGTGT)GATCCGGCACCAGCCCAGGAGGCTGTGGGAA NGG 10 8 17 8 24 0 100 5 10
    240605122312_s6oz AGATCCTCCTGCCTCTGCCTCCCAAGATCTGG(+CAGTGT)GATCCACACCCAGCTGTCCGTTATGTAAAAT NGG 10 8 17 8 24 0 100 5 10