Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605121819_yoxc TCCTCAGAAAATTGGACATAGTACTACCGGAG(+CAGTGT)GATCCAGCAATACCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10
    240605121817_zvju TTCTGGATATATGCCCAGGAGAGGTATTGCTG(+CAGTGT)GATCCTCCGGTAGTACTATGTCCAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240605121815_cs5f GGATGGACCATGTAGAGACTGCCATATCCAGG(+CAGTGT)GATCCACCCCATAATCAGCTTCCAAATGCTG NGG 10 8 17 8 24 0 100 5 10
    240605121814_21nu GGTGTCAGCATTTGGAAGCTGATTATGGGGTG(+CAGTGT)GATCCCTGGATATGGCAGTCTCTACATGGTC NGG 10 8 17 8 24 0 100 5 10
    240605121812_hzm2 AGAAGCATTCCTTGTCCTAGCCCTTCTCATTG(+CAGTGT)GTACCCTTGACTCTAAGCAGGGTCCCAAAGA NGG 10 8 17 8 24 0 100 5 10
    240605121810_zkxj AAGCCTCTTTGGGACCCTGCTTAGAGTCAAGG(+CAGTGT)GTACCAATGAGAAGGGCTAGGACAAGGAATG NGG 10 8 17 8 24 0 100 5 10
    240605121809_lmmk TGACCACTGAGATATGGATGATGGACTCCAAG(+CAGTGT)GATCCTTCAGCGGTATCTATTGTTGTTCTCT NGG 10 8 17 8 24 0 100 5 10
    240605121807_pe5w AAAACAGAGAACAACAATAGATACCGCTGAAG(+CAGTGT)GATCCTTGGAGTCCATCATCCATATCTCAGT NGG 10 8 17 8 24 0 100 5 10
    240605121806_46e9 GATTGAGCTCCAGCTATTCAGCTTGGCAGCAG(+CAGTGT)GTACCTTGAGCCCGAGCCACCTTGCTAGCAC NGG 10 8 17 8 24 0 100 5 10
    240605121805_a539 GACTTGTGCTAGCAAGGTGGCTCGGGCTCAAG(+CAGTGT)GTACCTGCTGCCAAGCTGAATAGCTGGAGCT NGG 10 8 17 8 24 0 100 5 10