Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605121803_mf08 TTTTGAAAGGTAAGTGTGACCAGAGGTGGCTG(+CAGTGT)GATCCAGATTTAAAATACACAACCATTAGCT NGG 10 8 17 8 24 0 100 5 10
    240605121802_owkw AACTCAGCTAATGGTTGTGTATTTTAAATCTG(+CAGTGT)GATCCAGCCACCTCTGGTCACACTTACCTTT NGG 10 8 17 8 24 0 100 5 10
    240605121801_reiw AAAAGAATATACCTTCTTCTCAGCACCTCATG(+CAGTGT)GTACCTTTTCCAAAACTGACCATGTAACTGG NGG 10 8 17 8 24 0 100 5 10
    240605121800_jwwf TGTGACCAGTTACATGGTCAGTTTTGGAAAAG(+CAGTGT)GTACCATGAGGTGCTGAGAAGAAGGTATATT NGG 10 8 17 8 24 0 100 5 10
    240605121759_b2dv AAGAAAATATGAGGCCAAGAATCTGGGGACAG(+CAGTGT)GATCCTTTTAGTCTCCATCTGAGCCTCAGAG NGG 10 8 17 8 24 0 100 5 10
    240605121757_ho75 GTTAACTCTGAGGCTCAGATGGAGACTAAAAG(+CAGTGT)GATCCTGTCCCCAGATTCTTGGCCTCATATT NGG 10 8 17 8 24 0 100 5 10
    240605121755_ysw6 GGATGGACCATGTAGAGACTGTCATATCCAGG(+CAGTGT)GATCCACCCCATAATCAGCATCCAAACGCTG NGG 10 8 17 8 24 0 100 5 10
    240605121753_3s4d GGTGTCAGCGTTTGGATGCTGATTATGGGGTG(+CAGTGT)GATCCCTGGATATGACAGTCTCTACATGGTC NGG 10 8 17 8 24 0 100 5 10
    240605121752_04wl CGTGGGTGGGGTCGATGGTCAGCTACATTGAG(+CAGTGT)GATCCAAGAATGGCAGCAGTGCCTGCACCCC NGG 10 8 17 8 24 0 100 5 10
    240605121751_4jhk CCAGTGGGGTGCAGGCACTGCTGCCATTCTTG(+CAGTGT)GATCCTCAATGTAGCTGACCATCGACCCCAC NGG 10 8 17 8 24 0 100 5 10