Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605121719_tc3b TAACAGGGCAGAGACTTGAACAGGATACCAGG(+CAGTGT)GTACCCAGCAAAACTGCCCTTTGATTGGCCT NGG 10 8 17 8 24 0 100 5 10
    240605121717_kbwi TAGTGAGGCCAATCAAAGGGCAGTTTTGCTGG(+CAGTGT)GTACCCTGGTATCCTGTTCAAGTCTCTGCCC NGG 10 8 17 8 24 0 100 5 10
    240605121716_g3j6 TGCCGCTGGGCTCCTGTCAGGTCCCTTCAGAG(+CAGTGT)GTACCTGTGTGTCTGTGCTCTTTGTAGCTCA NGG 10 8 17 8 24 0 100 5 10
    240605121714_ntau AGGGATGAGCTACAAAGAGCACAGACACACAG(+CAGTGT)GTACCTCTGAAGGGACCTGACAGGAGCCCAG NGG 10 8 17 8 24 0 100 5 10
    240605121713_i40v CCAGATCTTATGGAGGCATTTTCTCAATTGAG(+CAGTGT)GATCCCTCCTCTCAAATGACTGCAGCTTATA NGG 10 8 17 8 24 0 100 5 10
    240605121711_ipqm CTTGATATAAGCTGCAGTCATTTGAGAGGAGG(+CAGTGT)GATCCTCAATTGAGAAAATGCCTCCATAAGA NGG 10 8 17 8 24 0 100 5 10
    240605121710_v91k CACTCTGTGGACCAGGCTGGCCTCAAACTCAG(+CAGTGT)GATCCTCCTGAGTGCTGGGATTAAAGGCGTA NGG 10 8 17 8 24 0 100 5 10
    240605121708_w9a0 TGGTGTACGCCTTTAATCCCAGCACTCAGGAG(+CAGTGT)GATCCTGAGTTTGAGGCCAGCCTGGTCCACA NGG 10 8 17 8 24 0 100 5 10
    240605121707_xv8c GTAAGGACCGACTTGCGGGTAGCATAGTCTTG(+CAGTGT)GTACCAGATGATGTTTTTAGGGACATCAAAG NGG 10 8 17 8 24 0 100 5 10
    240605121706_ctk6 TGTTTCTTTGATGTCCCTAAAAACATCATCTG(+CAGTGT)GTACCAAGACTATGCTACCCGCAAGTCGGTC NGG 10 8 17 8 24 0 100 5 10