Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605121619_midc AGCTAGAGTACCCCTTCCCTCCCCACCACTGG(+CAGTGT)GATCCTTGATGGTACAGAGCAATTTTGCTTG NGG 10 8 17 8 24 0 100 5 10
    240605121618_gcib CTGCACAAGCAAAATTGCTCTGTACCATCAAG(+CAGTGT)GATCCCAGTGGTGGGGAGGGAAGGGGTACTC NGG 10 8 17 8 24 0 100 5 10
    240605121617_6m4p TTTTTTTTTTTTTTTTTTTTTTTTAAACTTGG(+CAGTGT)GATCCCTAGGGCAGCTTGCACAGGAAATCCT NGG 10 8 17 8 24 0 100 5 10
    240605121614_oopx ACCAAAGGATTTCCTGTGCAAGCTGCCCTAGG(+CAGTGT)GATCCCAAGTTTAAAAAAAAAAAAAAAAAAA NGG 10 8 17 8 24 0 100 5 10
    240605121612_ivvv GCTAACAACTATCTGTAACTCTACTCTAAAGG(+CAGTGT)GATCCGATGATCTCTGGCTCTACTGGGCACT NGG 10 8 17 8 24 0 100 5 10
    240605121611_b72w CATAAAGTGCCCAGTAGAGCCAGAGATCATCG(+CAGTGT)GATCCCTTTAGAGTAGAGTTACAGATAGTTG NGG 10 8 17 8 24 0 100 5 10
    240605121609_aw2l AAAATTGAAACTATAAACCAAGTACCCCACTG(+CAGTGT)GTACCCATCTTGTCTAAGCCCCTCTAACCTC NGG 10 8 17 8 24 0 100 5 10
    240605121607_3jhc TGAGGGAGGTTAGAGGGGCTTAGACAAGATGG(+CAGTGT)GTACCAGTGGGGTACTTGGTTTATAGTTTCA NGG 10 8 17 8 24 0 100 5 10
    240605121606_1atf GACTAGCTCATGTTAGCTCAGGCTCTGCTCAG(+CAGTGT)GATCCCAGCCAGCCCTGCTCTGCTCTGCTCT NGG 10 8 17 8 24 0 100 5 10
    240605121605_q44w AAGACAGAGCAGAGCAGAGCAGGGCTGGCTGG(+CAGTGT)GATCCTGAGCAGAGCCTGAGCTAACATGAGC NGG 10 8 17 8 24 0 100 5 10