Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605121510_6vbv TAATGGATAAGGCTTTATTTCTCTGCTGGAAG(+CAGTGT)GATCCATCTGGGAGAAAGAGAAGGGCAGGGG NGG 10 8 17 8 24 0 100 5 10
    240605121508_uwoc TATGGCCCCTGCCCTTCTCTTTCTCCCAGATG(+CAGTGT)GATCCTTCCAGCAGAGAAATAAAGCCTTATC NGG 10 8 17 8 24 0 100 5 10
    240605121507_uocs TCACAAAGGGGACCCCCTCCCAGGGCCTACAG(+CAGTGT)GATCCAGGGATTGAATGCTCAGGGAAGCACT NGG 10 8 17 8 24 0 100 5 10
    240605121505_w6wi GTGGTAGTGCTTCCCTGAGCATTCAATCCCTG(+CAGTGT)GATCCTGTAGGCCCTGGGAGGGGGTCCCCTT NGG 10 8 17 8 24 0 100 5 10
    240605121503_mio6 ACTACCAAATTGCTTGGCCCTTACCCTTCCAG(+CAGTGT)GATCCCTGCATATCCTCTCCAGCCCCAACCC NGG 10 8 17 8 24 0 100 5 10
    240605121502_bi3g GGGGTGGGTTGGGGCTGGAGAGGATATGCAGG(+CAGTGT)GATCCTGGAAGGGTAAGGGCCAAGCAATTTG NGG 10 8 17 8 24 0 100 5 10
    240605121500_zrwf TTGCTACTGGGCCACAGAATGGGTAACACGTG(+CAGTGT)GTACCAACTTTGATTTGGTCAAAGTTGATGT NGG 10 8 17 8 24 0 100 5 10
    240605121459_li2j AGGCAACATCAACTTTGACCAAATCAAAGTTG(+CAGTGT)GTACCACGTGTTACCCATTCTGTGGCCCAGT NGG 10 8 17 8 24 0 100 5 10
    240605121458_8byw TGCTACCCAAGGAGAGCATGCCAGATAGCGAG(+CAGTGT)GATCCAAGGTCAAGGCAGAGCCTTGACAGCT NGG 10 8 17 8 24 0 100 5 10
    240605121456_b0c6 CTCTTAGCTGTCAAGGCTCTGCCTTGACCTTG(+CAGTGT)GATCCTCGCTATCTGGCATGCTCTCCTTGGG NGG 10 8 17 8 24 0 100 5 10