Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605121439_so9v TTTTCAGACCAGCGACTCAGTTCATACAATGG(+CAGTGT)GATCCCAATTTTGTTGTAAAAATTTTTTGCC NGG 10 8 17 8 24 0 100 5 10
    240605121437_z5ml TTTTTGGCAAAAAATTTTTACAACAAAATTGG(+CAGTGT)GATCCCATTGTATGAACTGAGTCGCTGGTCT NGG 10 8 17 8 24 0 100 5 10
    240605121436_nsaw TCCTCCGTGGAGCTGTCTCTAGTGAGAATCAG(+CAGTGT)GTACCTGATTTGGTGAACCTGGATGGGAACC NGG 10 8 17 8 24 0 100 5 10
    240605121435_f9jh TCCCAGGTTCCCATCCAGGTTCACCAAATCAG(+CAGTGT)GTACCTGATTCTCACTAGAGACAGCTCCACG NGG 10 8 17 8 24 0 100 5 10
    240605121433_t4gl ATGGTGGACCGGCCACTGTGCCTATGCTATGG(+CAGTGT)GATCCCATCTCTTACAGCCCTGCAGGGAAGC NGG 10 8 17 8 24 0 100 5 10
    240605121432_ygpw CAAGAGCTTCCCTGCAGGGCTGTAAGAGATGG(+CAGTGT)GATCCCATAGCATAGGCACAGTGGCCGGTCC NGG 10 8 17 8 24 0 100 5 10
    240605121430_9vml TGTGGACCCCTACTGGGACGTGCTTGGTTTGG(+CAGTGT)GTACCTTCCTCGGTCCCACTGGCCGATTCTC NGG 10 8 17 8 24 0 100 5 10
    240605121428_na15 AAGTTGAGAATCGGCCAGTGGGACCGAGGAAG(+CAGTGT)GTACCCAAACCAAGCACGTCCCAGTAGGGGT NGG 10 8 17 8 24 0 100 5 10
    240605121427_ueuz CCACCTTGAAATCCCAAAGAAAGCAGTTCTTG(+CAGTGT)GTACCATCTATTATGGTTCTACCATTAAAAA NGG 10 8 17 8 24 0 100 5 10
    240605121426_avy3 CCCCTTTTTTAATGGTAGAACCATAATAGATG(+CAGTGT)GTACCAAGAACTGCTTTCTTTGGGATTTCAA NGG 10 8 17 8 24 0 100 5 10