Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605121338_9qnk ATTCTTTCACCAAGGAAGCAATGGTGGCCAGG(+CAGTGT)GTACCAGTCAGAGCCATTATTGCTCAGAGAA NGG 10 8 17 8 24 0 100 5 10
    240605121336_genk AGTAATTCTCTGAGCAATAATGGCTCTGACTG(+CAGTGT)GTACCCTGGCCACCATTGCTTCCTTGGTGAA NGG 10 8 17 8 24 0 100 5 10
    240605121335_w8x4 AGGAGTAATGTAAGGCTAGCTAAACTCTAGTG(+CAGTGT)GTACCAACGGAAGACTTCCGGTACGCCAGGC NGG 10 8 17 8 24 0 100 5 10
    240605121333_42kw ACCCCGCCTGGCGTACCGGAAGTCTTCCGTTG(+CAGTGT)GTACCACTAGAGTTTAGCTAGCCTTACATTA NGG 10 8 17 8 24 0 100 5 10
    240605121332_pxcr GTCCTTGGTTGTGAAGTATCATCCCAGTCATG(+CAGTGT)GTACCCTGTGCTGGCAACAGAAAAAAAAAAA NGG 10 8 17 8 24 0 100 5 10
    240605121330_xp0f TATTTTTTTTTTTTTTCTGTTGCCAGCACAGG(+CAGTGT)GTACCATGACTGGGATGATACTTCACAACCA NGG 10 8 17 8 24 0 100 5 10
    240605121328_7152 AGGCCTCACATCCCCACTCTGCTCCCCGCCTG(+CAGTGT)GTACCTGTGTTAATTTAGACCTTCACCACCT NGG 10 8 17 8 24 0 100 5 10
    240605121327_jemx GAGGCAGGTGGTGAAGGTCTAAATTAACACAG(+CAGTGT)GTACCAGGCGGGGAGCAGAGTGGGGATGTGA NGG 10 8 17 8 24 0 100 5 10
    240605121325_nsev ATAAGTCCCCATTTGAGGTCTAGGTTCCCAGG(+CAGTGT)GATCCATTTACCTGGCCAGCCAGAAGCCCCT NGG 10 8 17 8 24 0 100 5 10
    240605121323_ptp9 TATGCAGGGGCTTCTGGCTGGCCAGGTAAATG(+CAGTGT)GATCCCTGGGAACCTAGACCTCAAATGGGGA NGG 10 8 17 8 24 0 100 5 10