Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605121239_dfre GGCAACTAGCTTACAGTGAAGAGGAGGCTGAG(+CAGTGT)GATCCAAGCTCAGGCACTCTAGGCTACAAAA NGG 10 8 17 8 24 0 100 5 10
    240605121237_av9m ACCCCTTTTGTAGCCTAGAGTGCCTGAGCTTG(+CAGTGT)GATCCTCAGCCTCCTCTTCACTGTAAGCTAG NGG 10 8 17 8 24 0 100 5 10
    240605121236_xf78 CCCTGCCTTCACTGTAAAAGATCCCTAGGCAG(+CAGTGT)GATCCTCTCACTACATATCCCCAAACTATAG NGG 10 8 17 8 24 0 100 5 10
    240605121235_uo2x GAGTCCTATAGTTTGGGGATATGTAGTGAGAG(+CAGTGT)GATCCTGCCTAGGGATCTTTTACAGTGAAGG NGG 10 8 17 8 24 0 100 5 10
    240605121233_f9y3 GGATGGACCATCTAGAGACTGCCATATCCAGG(+CAGTGT)GATCCATCCCATAATTAGCCTCCAAAGGATG NGG 10 8 17 8 24 0 100 5 10
    240605121231_s0yr GGTGTCATCCTTTGGAGGCTAATTATGGGATG(+CAGTGT)GATCCCTGGATATGGCAGTCTCTAGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240605121230_w1ql ATTAGTAAGTCTAAAACACAAATAATTATAAG(+CAGTGT)GATCCGGAGTTTATATCTTTTCAAGAGCTAT NGG 10 8 17 8 24 0 100 5 10
    240605121228_geo2 TACAGATAGCTCTTGAAAAGATATAAACTCCG(+CAGTGT)GATCCTTATAATTATTTGTGTTTTAGACTTA NGG 10 8 17 8 24 0 100 5 10
    240605121226_odot TTCTGGATATATGCCCAGGAGAGGTATTGTGG(+CAGTGT)GATCCTCCAGTAGTACTATGTCCAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240605121225_852f TCCTTAGAAAATTGGACATAGTACTACTGGAG(+CAGTGT)GATCCCACAATACCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10